PFAS Antibody - Primary antibody, specific to PFAS, Rabbit IgG

Cat. No.: Ab121397

GRADE & PURITY Validated, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human PFAS Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/2000

IHC: 1/50-1/300

IF/ICC: 1/200
Relevance
Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. PFAS catalyzes the fourth step of IMP biosynthesis.

Specifications

Product Name

PFAS Antibody - Primary antibody, specific to PFAS, Rabbit IgG

Synonyms

PFAS antibody | FGAM synthase antibody | FGAMS antibody | FGAR amidotransferase antibody | FGARAT antibody | Formylglycinamide ribotide amidotransferase antibody | Formylglycinamide ribotide synthetase antibody | KIAA0361 antibody | Phosphoribosylformylgl

Specifications & Purity

Validated, 1.0 mg/mL

Host species

Rabbit

Specificity

PFAS

Immunogen

A synthetic peptide derived from human PUR4 (AA 73-123).

Positive Control

WB: HeLa and HEK293 cell lysates. IHC: Human tonsil tissues. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: PFAS Antibody (Ab121397)

PFAS Antibody (Ab121397) - Western Blot
All lanes: PFAS Antibody (Ab121397) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 145 kDa
Observed band size: 133 kDa
Exposure time: 120.1 s

IHC: PFAS Antibody (Ab121397)

PFAS Antibody (Ab121397) - IHC
Immunohistochemistry analysis of paraffin-embedded human tonsil labelling PFAS with PFAS Antibody (Ab121397) at 1/200 dilution (4℃, overnight). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was used for antigen retrieval.​ Secondary antibody was diluted at 1/200 dilution (room temperature, 45min).

IF/ICC: PFAS Antibody (Ab121397)

PFAS Antibody (Ab121397) - IF/ICC 

IF analysis of PFAS Antibody (Ab121397) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PFAS Antibody (Ab121397) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IF/ICC:

  1/200;

• IHC:

  1/50-1/300 ;

Associated Targets(Human)

PFAS Tbio Phosphoribosylformylglycinamidine synthase (0 Activities)

Discover Target: PFAS

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

References

1. Na Liu, Yinhui Li, Minggu Zhang, Naiju Che, Xianliang Song, Yanli Liu, Chengliang Li.  (2024)  Efficient adsorption of short-chain perfluoroalkyl substances by pristine and Fe/Cu-loaded reed straw biochars.  SCIENCE OF THE TOTAL ENVIRONMENT,      [PMID:38917893] [10.1016/j.scitotenv.2024.174223]