Phosphate acetyltransferase (PTA） , CAS No.9029-91-8

CAS: 9029-91-8 Cat. No.: P1431341 Número EC: 232-855-2

CALIDAD & PUREZA Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥3000 U/mL; ~3500 U/mg protein

Accession #

P39646

Bioactivity

≥3000 U/mL; ~3500 U/mg protein

Descripción general

High purity recombinant phosphotransacetylase (Bacillus subtilis) for use in research, biochemical enzyme assays and analytical testing applications. 

Specificity: 

Catalyses the reaction: Coenzyme A + acetyl phosphate = acetyl-CoA + phosphate

Temperature Optima: 25°C

pH Optima: 7.4

Application examples: Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.

PROPERTIES 

1. ELECTROPHORETIC PURITY: 

- Single band on SDS-gel electrophoresis (MW ~ 36,953) 

- Single major band on isoelectric focusing (pI ~ 5.3) 

2. PHYSICOCHEMICAL PROPERTIES: Recommended conditions of use are at pH 7.4 and up to 25°C. 

3. STORAGE AND USE CONDITIONS/RECOMMENDATIONS: The enzyme is supplied as an ammonium sulphate suspension containing 0.5 mg/mL BSA and should be stored at 4°C. For assay, this enzyme should be diluted in 100 mM Tris.HCl buffer, pH 7.4 containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use. 

Especificaciones

Product Name

Phosphate acetyltransferase (PTA） , CAS No.9029-91-8

Sinónimos

Acetyl-CoA:orthophosphate acetyltransferase | phosphate acetyltransferase | phosphoacylase | phosphotransacetylase | PTA

Grado

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Especificaciones y pureza

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥3000 U/mL; ~3500 U/mg protein

Bioactividad

≥3000 U/mL; ~3500 U/mg protein

Número de adhesión

P39646

Peso molecular previsto

36,953

CAS

9029-91-8

Número de la Comisión de Enzimas

EC 2.3.1.8

Tipo de molécula

Enzima

Product Line

Productos biológicos

Almacenamiento y envío

Concentración

≥3000 U/mL; ~3500 U/mg protein

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Estabilidad y almacenamiento

Store at 2-8℃ long term. Upon receipt, it is recommended to aliquot.

Definición de unidad

One Unit of phosphotransacetylase activity is defined as the amount of enzyme required to release one µmole of acetyl-CoA per minute from Coenzyme A (0.44 mM) in Tris.HCl buffer (86 mM) pH 7.4 at 25°C.