PPP2R5A Antibody - Anticuerpo primario, specific to PPP2R5A, Rabbit IgG

Cat. No.: Ab122866

GRADE & PURITY Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IF/ICC, WB

Host species

Conejo

Reactive species

Cerdo, consejo, Humano, Mono, Ratón, Vaca

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human PPP2R5A Antibody, Polyclonal, could be used for WB, ICC/IF and so on.
Application
WB: 1/500-1/1000
ICC/IF: 1/100-1/500
Protein Function
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Specifications

Product Name

PPP2R5A Antibody - Anticuerpo primario, specific to PPP2R5A, Rabbit IgG

Sinónimos

B56A antibody | 2A5A_HUMAN antibody | MGC131915 antibody | PP2A B subunit B' alpha isoform antibody | PP2A B subunit B56 alpha isoform antibody | PP2A B subunit isoform B''-alpha antibody | PP2A B subunit isoform B56-alpha antibody | PP2A B subunit isofor

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Especies hospedadoras

Conejo

Especificidad

PPP2R5A

Inmunógeno

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PPP2R5A.

Control positivo

WB: Mouse skeletal muscle, Jurkat and NIH/3T3 lysates. IF/ICC: NIH/3T3 cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Immunogen affinity purified

Concentración

1.0 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: PPP2R5A Antibody (Ab122866)

PPP2R5A Antibody (Ab122866) - Western Blot
All lanes: PPP2R5A Antibody (Ab122866) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 56 kDa
Observed band size: 52 kDa
Exposure time: 266.0 s

IF/ICC: PPP2R5A Antibody (Ab122866)

PPP2R5A Antibody (Ab122866) - IF/ICC
Immunofluorescent analysis of PPP2R5A staining in NIH/3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with PPP2R5A Antibody (Ab122866) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Aplicación

Aplicación

Dilution info

WB

1/500-1/1000

ICC/IF

1/100-1/500

Objetivos asociados (humanos)

PPP2R5A Tchem Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform (0 Activities)

Discover Target: PPP2R5A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PPP2R5A Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.