PSMD1 Mouse mAb - Anticuerpo primario, specific to PSMD1, Mouse IgG1

Cat. No.: Ab123682

CALIDAD & PUREZA Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, WB

Host species

Ratón

Reactive species

consejo, Humano, Ratón

Isotype

Mouse IgG1

Clone Number

K70027_8C11

Conjugation

Unconjugated

Descripción general

Mouse anti Human PSMD1 Antibody, Monoclonal (K70027_8C11), could be used for WB, IHC and so on.
Application:
WB: 1/1000
IHC: 1/200 
Protein Function

Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

Especificaciones

Product Name

PSMD1 Mouse mAb - Anticuerpo primario, specific to PSMD1, Mouse IgG1

Sinónimos

MGC133041 antibody | 26S proteasome non ATPase regulatory subunit 1 antibody | 26S proteasome non ATPase subunit 1 antibody | 26S proteasome non-ATPase regulatory subunit 1 antibody | 26S proteasome regulatory subunit rpn2 antibody | 26S proteasome regula

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Especies hospedadoras

Ratón

Especificidad

PSMD1

Inmunógeno

Recombinant protein of human PSMD1 (AA 791-920).

Control positivo

WB: Hep G2, HeLa, Jurkat, MCF7, A431, SK-OV-3, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -80°C,Avoid repeated freezing and thawing

Enviado en

Dry ice packs + Cold packs

Estabilidad y almacenamiento

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: PSMD1 Mouse mAb (Ab123682)

PSMD1 Mouse mAb (Ab123682) - Western Blot
All lanes: PSMD1 Mouse mAb (Ab123682) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 108 kDa
Exposure time: 45.7 s

IHC: PSMD1 Mouse mAb (Ab123682)

PSMD1 Mouse mAb (Ab123682) - IHC
IHC-P analysis of testis tissue by PSMD1 Mouse mAb (Ab123682).IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with PSMD1 Mouse mAb (Ab123682) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in seminiferous ducts are positively stained at the nuclei and cytoplasm.

Aplicación

Aplicación

Dilution info

WB

1/1000

IHC

1/200

Objetivos asociados (humanos)

PSMD1 Tbio 26S proteasome non-ATPase regulatory subunit 1 (0 Activities)

Discover Target: PSMD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PSMD1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.