Puromycin Hydrochloride Solution (1 mg/mL) - BioReagent,for cell culture,1 mg/ml , CAS No.58-58-2

CAS: 58-58-2 Cat. No.: P1509572 Fórmula: C₂₂H₂₉N₇O₅·2HCl Peso molecular: 544.44 Beilstein Registry Number: 3853613 Número EC: 200-387-8

GRADE & PURITY BioReagent, for Cell culture, 1 mg/ml

Synonyms

Puromycin Hydrochloride Solution

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Culture

Descripción general

  Puromycin (PM) is a protein synthesis inhibitor with a CAS number of 53-79-2. It has a structure similar to the terminal end of tRNA molecules, which enables it to bind to amino acids, replace aminoacylated tRNA to bind to the A-site of ribosomes, and be incorporated into the growing peptide chain. Although puromycin can bind to the A-site, it cannot participate in any subsequent reactions, thus terminating protein synthesis and releasing immature polypeptides containing puromycin at the C-terminus. Puromycin dihydrochloride is an aminonucleoside antibiotic produced by the fermentation of Streptomyces alboniger. As a stable hydrochloride form of puromycin, it is often referred to as puromycin hydrochloride, with a CAS number of 58-58-2.

  Puromycin Hydrochloride Solution (1 mg/mL) is formulated with high-purity raw materials and methanol. If necessary, it should be filtered through an organic filter membrane before being used for cell culture. Its molar concentration is 1.84 mmol/L, and the general working concentration ranges from 10 to 100 μmol/L. This product is for research use only (RUO) and is not intended for clinical diagnosis or any other applications.

Operating Procedures (For Reference Only)

1. Operate in accordance with specific experimental requirements.

2. Taking a working concentration of 100 μmol/L as an example, add 544 μL of  Puromycin Hydrochloride Solution (1 mg/mL) to every 10 mL of culture medium.

Precautions

1. Ensure sterile operation to avoid contamination. Minimize repeated freeze-thaw cycles to prevent reagent inactivation.

2. For your safety and health, wear a lab coat and disposable gloves during operation.

Specifications

Sinónimos

Puromycin Hydrochloride Solution

Especificaciones y pureza

BioReagent,for cell culture,1 mg/ml

Estabilidad y almacenamiento

Store at -20℃ long term (12 months). Store in the dark.

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -20°C

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent, para el cultivo celular

Nombres e identificadores

• Isómeros SMILES: CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl
• WGK Alemania: 3
• RTECS: AU7355000
• CAS alternativo: 53-79-2
• Peso molecular: 544.44
• Beilstein: 3853613
• Reaxy-Rn: 39769009
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=39769009&ln=

Propiedades químicas y físicas

• Sensibilidad: Light-sensitive
• Punto de fusión (°C): 174°C(lit.)

Referencias

1. Jing Zhou, Tongyu Wu, Chun Li, Zhuozhou Hu, Liang Han, Xiangxiang Li, Jingjing Liu, Wenyang Zhao, Jia Kang, Xinping Chen.  (2023)  Alfuzosin ameliorates diabetes by boosting PGK1 activity in diabetic mice.  LIFE SCIENCES,      [PMID:36758669] [10.1016/j.lfs.2023.121491]

2. Song Runjie, Ma Shuoqian, Xu Jiajia, Ren Xin, Guo Peilan, Liu Huijiao, Li Peng, Yin Fan, Liu Mei, Wang Qiang, Yu Lei, Liu Jiali, Duan Binwei, Rahman Nafis A., Wołczyński Sławomir, Li Guangming, Li Xiangdong.  (2023)  A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR.  Molecular Cancer,  22  (1): (1-20).  [PMID:36691031] [10.1186/s12943-023-01719-9]

3. Zhuozhou Hu, Jing Zhou, Liang Han, Xiangxiang Li, Chun Li, Tongyu Wu, Jingjing Liu, Wenyang Zhao, Jia Kang, Xinping Chen.  (2022)  Acyclovir alleviates insulin resistance via activating PKM1 in diabetic mice.  LIFE SCIENCES,      [PMID:35751919] [10.1016/j.lfs.2022.120725]

4. Qiong Yang, Maochen Xing, Ke Wang, Qiang Wei, Jiarui Zhao, Yuan Wang, Kai Ji, Shuliang Song.  (2022)  Application of Fucoidan in Caco-2 Model Establishment.  Pharmaceuticals,  15  (4): (418).  [PMID:35455415] [10.3390/ph15040418]

5. Bo Zhang, Ting Jiang, Li Ling, Zhonglian Cao, Jingjing Zhao, Yanyan Tuo, Xiaojian She, Shun Shen, Xinguo Jiang, Yu Hu, Zhiqing Pang.  (2016)  Enhanced Antitumor Activity of EGFP-EGF1-Conjugated Nanoparticles by a Multitargeting Strategy.  ACS Applied Materials & Interfaces,      [PMID:26890991] [10.1021/acsami.6b00036]

6. Jia Liu, Liuting Zheng, Xinyue Li, Wei Tang, Manyu Guo, Yuxing Wang, Xiaoqi Tan, Jiajia Chang, Huiyue Zhao, Dongsheng Zhu, Yu-qiang Ma, Da Huo.  (2025)  Emerging of Ultrafine Membraneless Organelles as the Missing Piece of Nanostress: Mechanism of Biogenesis and Implications at Multilevels.  ACS Nano,      [PMID:39882824] [10.1021/acsnano.4c15876]

7. Zikang Xing, Xuewen Li, Zhen Ning Tony He, Xin Fang, Heng Liang, Chunxiang Kuang, Aiying Li, Qing Yang.  (2024)  IDO1 Inhibitor RY103 Suppresses Trp-GCN2-Mediated Angiogenesis and Counters Immunosuppression in Glioblastoma.  Pharmaceutics,  16  (7): (870).  [PMID:39065567] [10.3390/pharmaceutics16070870]

8. Ping Yang, Zheng-Jian Wang, Hai-Tao Lu, Xu-Meng Feng, Jing-Long Ye, Guangchuan Wang, Cheng-Feng Qin, Qing Ye, Zhong-Yu Liu.  (2024)  Imaging of viral replication in live cells by using split fluorescent protein-tagged reporter flaviviruses.  VIROLOGY,      [PMID:39754862] [10.1016/j.virol.2024.110374]

9. Xinrui Meng, Jingjing Liu, Jia Kang, Menghan Wang, Zhanghui Guan, Dong Tian, Xinping Chen.  (2024)  Lamivudine protects mice from gastric ulcer by activating PGK1 to suppress ferroptosis.  BIOCHEMICAL PHARMACOLOGY,      [PMID:39029631] [10.1016/j.bcp.2024.116440]

10. Hui-Yue Zhao, Yuan-Qiang Chen, Xing-Yu Luo, Ming-Jie Cai, Jia-Yi Li, Xin-Yu Lin, Hao Zhang, Hong-Ming Ding, Guang-Liang Jiang, Yong Hu.  (2024)  Ligand Phase Separation-Promoted, “Squeezing-Out” Mode Explaining the Mechanism and Implications of Neutral Nanoparticles That Escaped from Lysosomes.  ACS Nano,      [PMID:38198577] [10.1021/acsnano.3c09452]

11. Jingxin Sun, Zhehao Jin, Yong Jin, Haidan Yuan, Guangyu Jin, Jishan Quan.  (2025)  MRI-Based Multifunctional Nanoliposomes for Enhanced HCC Therapy and Diagnosis.  MOLECULAR PHARMACEUTICS,      [PMID:39809473] [10.1021/acs.molpharmaceut.4c00917]

12. Wei Li, Qingwen Zeng, Bing Wang, Chao Lv, Haoan He, Xi Yang, Bin Cheng, Xiaoan Tao.  (2024)  Oxidative stress promotes oral carcinogenesis via Thbs1-mediated M1-like tumor-associated macrophages polarization.  Redox Biology,      [PMID:39255693] [10.1016/j.redox.2024.103335]

13. Yao Yongchang, Chen Ke, Pan Qian, Gao Hui, Su Weixian, Zheng Shicong, Dong Weiqiang, Qian Dongyang.  (2024)  Redifferentiation of genetically modified dedifferentiated chondrocytes in a microcavitary hydrogel.  BIOTECHNOLOGY LETTERS,      [PMID:38523201] [10.1007/s10529-024-03475-2]

14. Xiuli Zhang, Mingjing Wei, Zhigang Zhang, Yarong Zeng, Feihong Zou, Sibo Zhang, Zhiping Wang, Fentian Chen, Hualong Xiong, Yufang Li, Lizhi Zhou, Tingting Li, Qingbing Zheng, Hai Yu, Jun Zhang, Ying Gu, Qinjian Zhao, Shaowei Li, Ningshao Xia.  (2024)  Risedronate-functionalized manganese-hydroxyapatite amorphous particles: A potent adjuvant for subunit vaccines and cancer immunotherapy.  JOURNAL OF CONTROLLED RELEASE,      [PMID:38244843] [10.1016/j.jconrel.2024.01.033]

15. Zheng Liu-Ting, Yan Zeng-Shuai, Li Xin-Yue, Chang Jia-Jia, Tan Xiao-Qi, Wang Yu-Xing, Ding Hong-Ming, Liu Qin, Ma Yu-Qiang, Huo Da.  (2025)  Nanomaterial signatures program biomolecular condensates via triphasic separation for chemoplasticity remodeling.  Nature Communications,  16  (1): (1-24).  [PMID:41162367] [10.1038/s41467-025-64623-4]