RBP1 Mouse mAb - Anticuerpo primario, specific to RBP1

Cat. No.: Ab124812

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Ratón

Reactive species

consejo, Humano, Ratón

Isotype

Mouse IgG2a

Clone Number

OTI2H3

Conjugation

Unconjugated

Descripción general

Mouse anti Human RBP1 Antibody, Monoclonal (OTI2H3), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

RBP1 Mouse mAb - Anticuerpo primario, specific to RBP1

Sinónimos

Cellular retinol binding protein antibody | Cellular retinol-binding protein antibody | Cellular retinol-binding protein I antibody | CRABP I antibody | CRBP antibody | CRBP-I antibody | CRBP1 antibody | CRBPI antibody | RBP 1 antibody | RBP1 antibody | R

Especificaciones y pureza

ExactAb™, Validated, See COA

Mecanismos bioquímicos y fisiológicos

Cytoplasmic retinol-binding protein. Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis.

Especies hospedadoras

Ratón

Especificidad

RBP1

Inmunógeno

Recombinant full length protein of human RBP1.

Control positivo

WB: Rat brain, NIH/3T3 lysates. IHC: Human liver, ovary, ovary adenocarcinoma, endometrium tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY RBP1. Flow Cytometry: HEK293T cells transfected with pCMV6-ENTRY RBP1.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: RBP1 Mouse mAb (Ab124812)

RBP1 Mouse mAb (Ab124812) - Western Blot
All lanes: RBP1 Mouse mAb (Ab124812) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 140.1 s

IHC: RBP1 Mouse mAb (Ab124812)

RBP1 Mouse mAb (Ab124812) - IHC
Immunohistochemical staining of paraffinembedded Human liver tissue within the normal limits using RBP1 Mouse mAb (Ab124812). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: RBP1 Mouse mAb (Ab124812)

RBP1 Mouse mAb (Ab124812) - IHC
Immunohistochemical staining of paraffinembedded Human Ovary tissue within the normal limits using RBP1 Mouse mAb (Ab124812). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: RBP1 Mouse mAb (Ab124812)

RBP1 Mouse mAb (Ab124812) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human ovary tissue using RBP1 Mouse mAb (Ab124812). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: RBP1 Mouse mAb (Ab124812)

RBP1 Mouse mAb (Ab124812) - IHC
Immunohistochemical staining of paraffinembedded Human endometrium tissue within the normal limits using RBP1 Mouse mAb (Ab124812). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IF/ICC: RBP1 Mouse mAb (Ab124812)

RBP1 Mouse mAb (Ab124812) - IF/ICC
RBP1 Mouse mAb (Ab124812) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RBP1.

IF/ICC: RBP1 Mouse mAb (Ab124812)

RBP1 Mouse mAb (Ab124812) - IF/ICC
Immunofluorescent staining of HeLa cells using RBP1 Mouse mAb (Ab124812).

Flow: RBP1 Mouse mAb (Ab124812)

RBP1 Mouse mAb (Ab124812) - Flow
HEK293T cells transfected with either pCMV6-ENTRY RBP1 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by RBP1 Mouse mAb (Ab124812), and then analyzed by flow cytometry.

Flow: RBP1 Mouse mAb (Ab124812)

RBP1 Mouse mAb (Ab124812) - Flow
Flow cytometric Analysis of Hela cells, using RBP1 Mouse mAb (Ab124812), (Red), compared to a nonspecific negative control antibody, (Blue).

Flow: RBP1 Mouse mAb (Ab124812)

RBP1 Mouse mAb (Ab124812) - Flow
Flow cytometric Analysis of Jurkat cells, using RBP1 Mouse mAb (Ab124812), (Red), compared to a nonspecific negative control antibody, (Blue).

Aplicación

• Aplicación: Dilution info
• WB:

  1/2000;

• Flow Cytometry:

  1/100;

• IF/ICC:

  1/100;

• IHC:

  1/150

Objetivos asociados (humanos)

RBP1 Tbio Retinol-binding protein 1 (0 Activities)

Discover Target: RBP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RBP1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.