RCAS1 Mouse mAb - Primary antibody, specific to RCAS1, Mouse IgG1

Cat. No.: Ab124859

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

K40051_4H7

Conjugation

Unconjugated

Overview

Mouse anti Human RCAS1 Antibody, Monoclonal (K40051_4H7), could be used for WB, IHC and so on.
Application:
WB:1/1000
IHC:1/100
Protein Function:
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.

Specifications

Product Name

RCAS1 Mouse mAb - Primary antibody, specific to RCAS1, Mouse IgG1

Synonyms

PDAF antibody | RCAS 1 antibody | RCAS1 antibody | RCAS1_HUMAN antibody | Receptor binding cancer antigen expressed on SiSo cells antibody | Receptor-binding cancer antigen expressed on SiSo cells antibody | EBAG9 | EB9 | PDAF | BAG9 antibody | Cancer ass

Specifications & Purity

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Host species

Mouse

Specificity

RCAS1

Immunogen

Recombinant human EBAG9 (AA 41-210).

Positive Control

WB: SK-OV-3, MCF7, A549, Jurkat, HEK293, A431, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Stability And Storage

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: RCAS1 Mouse mAb (Ab124859)

RCAS1 Mouse mAb (Ab124859) - Western Blot
All lanes: RCAS1 Mouse mAb (Ab124859) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 31 kDa
Exposure time: 36.5 s

IHC: RCAS1 Mouse mAb (Ab124859)

RCAS1 Mouse mAb (Ab124859) - IHC
IHC analysis of human brain tissue by RCAS1 Mouse mAb (Ab124859). IHC was performed using sections of the formalin-fixed paraffin-embedded human brain tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with RCAS1 Mouse mAb (Ab124859) at 10 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glial cells and neuronal cells are positively stained at the cytoplasm.

Application

• Application: Dilution info
• WB:

  1/1000 ;

• IHC:

  1/100;

Associated Targets(Human)

EBAG9 Tbio Receptor-binding cancer antigen expressed on SiSo cells (0 Activities)

Discover Target: EBAG9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)