Recombinant CD16 Antibody - Anticuerpo primario, specific to Human CD16

Cat. No.: Ab212149

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), See COA

Application

ELISA, Flow Cytometry

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

P93C38

Conjugation

Unconjugated

Descripción general

Recombinant CD16 Antibody, clone P93C38, could be used for Flow Cytometry, ELISA and so on.

Specifications

Product Name

Recombinant CD16 Antibody - Anticuerpo primario, specific to Human CD16

Sinónimos

CD16 | CD16A | Fc fragment of IgG receptor IIIa | Fc gamma RIII | FCG3 | FCGR3 | FcgRIII | FCR-10 | FCRIII | FCRIIIA | IGFR3 | IMD20 | CD 16 antibody | CD 16a antibody | CD16 antibody | CD16a antibody | CD16a antigen antibody | CD16B antibody | CD16b anti

Especificaciones y pureza

Animal Free,Carrier Free,Recombinant,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),See COA

Mecanismos bioquímicos y fisiológicos

Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molec

Especies hospedadoras

Ratón

Especificidad

Human CD16

Inmunógeno

Human polymorphonuclear leukocytes

Control positivo

Flow Cytometry: Human peripheral blood lymphocytes

Conjugación

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Recombinant, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Flow Cytometry: Recombinant CD16 Antibody (Ab212149)

Recombinant CD16 Antibody (Ab212149) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD16 with Recombinant CD16 Antibody (Ab212149) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Mouse IgG (Biotin) (Ab180306) - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: Recombinant CD16 Antibody (Ab212149)

Recombinant CD16 Antibody (Ab212149) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD16 (red) with Recombinant CD16 Antibody (Ab212149) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab180306) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

SEC: Recombinant CD16 Antibody (Ab212149)

Recombinant CD16 Antibody (Ab212149) - SEC
The purity of Recombinant CD16 Antibody (Ab212149) is more than 95% verified by HPLC.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.1 µg per million cells in 100 µL volume;

Objetivos asociados (humanos)

FCGR3A Tclin Low affinity immunoglobulin gamma Fc region receptor III-A (0 Activities)

Discover Target: FCGR3A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CD16 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.