Recombinant CD19 Antibody - Anticuerpo primario, specific to Human CD19

Cat. No.: Ab219599

CALIDAD & PUREZA Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), See COA

Application

ELISA, Flow Cytometry

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

P08D14

Conjugation

Unconjugated

Descripción general

Recombinant CD19 Antibody, clone P08D14, could be used for Flow Cytometry, ELISA and so on.

Especificaciones

Product Name

Recombinant CD19 Antibody - Anticuerpo primario, specific to Human CD19

Sinónimos

B4 | B-lymphocyte antigen CD19 | B-lymphocyte surface antigen B4 | CD19 antigen | CD19 molecule | CD19 | CVID3 | Differentiation antigen CD19 | Leu-12 | MGC12802 | T-cell surface antigen Leu-12 | Antibody deficiency due to defect in CD19 antibody | Antibo

Especificaciones y pureza

Animal Free,Carrier Free,Recombinant,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),See COA

Mecanismos bioquímicos y fisiológicos

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to th

Especies hospedadoras

Ratón

Especificidad

Human CD19

Inmunógeno

Human Chronic Lymphocytic Leukemia (CLL) Cells.

Control positivo

Flow Cytometry: Human peripheral blood lymphocytes

Conjugación

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Recombinant, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Flow Cytometry: Recombinant CD19 Antibody (Ab219599)

Recombinant CD19 Antibody (Ab219599) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphcytes labelling CD19 with Recombinant CD19 Antibody (Ab219599) (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Ab170221) (left panel) and detected with AF 488-conjugated affinipure goat anti-Mouse IgG.

Flow Cytometry: Recombinant CD19 Antibody (Ab219599)

Recombinant CD19 Antibody (Ab219599) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphcytes labelling CD19 (red) with Recombinant CD19 Antibody (Ab219599) at 0.5 μg/mL for 1 hour at 4°C, and detected with AF 488-conjugated affinipure goat anti-Mouse IgG. Blue - Mouse IgG Isotype Control Antibody (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Recombinant CD19 Antibody (Ab219599)

Recombinant CD19 Antibody (Ab219599) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD19 (red) with Recombinant CD19 Antibody (Ab219599) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

SEC: Recombinant CD19 Antibody (Ab219599)

Recombinant CD19 Antibody (Ab219599) - SEC
The purity of Recombinant CD19 Antibody (Ab219599) is more than 95% verified by HPLC.

Aplicación

Aplicación

Dilution info

Flow Cytometry

≤0.1 µg per million cells in 100 µL volume

Objetivos asociados (humanos)

CD19 Tclin B-lymphocyte antigen CD19 (0 Activities)

Discover Target: CD19

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CD19 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.