GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.5 μL/test
Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells.
The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation a
Especies hospedadoras
Ratón
Especificidad
CD27
Inmunógeno
Human Thymocytes
Control positivo
Flow Cytometry: Human peripheral blood lymphocytes
Conjugación
AF488
Excitación (nm)
Blue Laser(488nm)
Emisión (nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
Grado
ExactAb™, Recombinant, Validated
Tipo de cadena ligera
kappa
Método de purificación
Protein A purified
Concentración
5 μL/test
Product Line
Productos biológicos
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD27 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD27 Antibody (AF488) (Ab232002) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).
Flow cytometry analysis of CD27 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD27 Antibody (AF488) (Ab232002) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.
Aplicación
Aplicación
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Reseñas
Reseñas de cliente
Preguntas frecuentes
How should this product be stored?
Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.
How is this product shipped?
This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CD27 Antibody (AF488)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
Utilizamos cookies para garantizar el correcto funcionamiento del sitio web y, cuando esté permitido, para mejorar su experiencia. Puede gestionar sus preferencias en cualquier momento en Configuración. Más información en nuestra Política de Cookies.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ25F1129494