Recombinant eIF2A Antibody - Anticuerpo primario, specific to EIF2A, Rabbit IgG

Cat. No.: Ab101265

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, WB

Host species

Conejo

Reactive species

consejo, Humano, Ratón

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human eIF2A Antibody, Recombinant, could be used for WB and ICC/IF.
Application:
WB: 1/500 – 1/1000
ICC/IF: 1/50 – 1/200
Protein Function
Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.

Specifications

Product Name

Recombinant eIF2A Antibody - Anticuerpo primario, specific to EIF2A, Rabbit IgG

Sinónimos

65 kDa eukaryotic translation initiation factor 2A antibody | CDA 02 antibody | CDA02 antibody | EIF 2 antibody | EIF 2A antibody | eIF-2A antibody | EIF2 antibody | eif2a antibody | EIF2A_HUMAN antibody | Eukaryotic translation initiation factor 2A 65kDa

Especificaciones y pureza

ExactAb™, Validated, Recombinant, 0.5 mg/mL

Especies hospedadoras

Conejo

Especificidad

EIF2A

Inmunógeno

A synthetic peptide derived from human eIF2A (AA 480-494).

Control positivo

WB: MCF7, A549, HepG2, K562, A431 and NIH/3T3 cell lysates; ICC/IF: MCF7.

Conjugación

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Antigen affinity purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Recombinant eIF2A Antibody (Ab101265)

Recombinant eIF2A Antibody (Ab101265) - Western Blot
All lanes: Recombinant eIF2A Antibody (Ab101265) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 65 kDa
Observed band size: 65&55 kDa
Exposure time: 1.7 s

IF: Recombinant eIF2A Antibody (Ab101265)

Recombinant eIF2A Antibody (Ab101265) - IF
Immunocytochemistry analysis of eIF2A (green) in MCF7 using Recombinant eIF2A Antibody (Ab101265) and cells were counterstained with DAPI (blue).

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

Objetivos asociados (humanos)

EIF2A Tbio Eukaryotic translation initiation factor 2A (0 Activities)

Discover Target: EIF2A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant eIF2A Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.