Recombinant Human FGF basic/FGF2/bFGF Protein, ≥95% (SDS-PAGE), CAS No.62031-54-3

CAS: 62031-54-3 Cat. No.: rp155946 Molecular Weight: 17kD

GRADE & PURITY Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, ≥95%(SDS-PAGE)

Expression System

Oryza sativa

Accession #

P09038

Protein Tag

No tag

Expression system

Oryza sativa

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.

Overview

Basic fibroblast growth factor (bFGF, FGF2 or FGF-β) is a member of the fibroblast growth factor family. bFGF is a kind of single-chain polypeptide growth factor, which plays an important role in promoting cell proliferation. It can also inhibit cell apoptosis, accelerate wound healing and induce blood vessel regeneration. In many cell types, such as mesoderm and neuroectoderm, bFGF is also very effective in inducing DNA synthesis. OsrhbFGF is expressed by rice endosperm cells and purified by gene recombination technology.

Specifications

Product Name

Recombinant Human FGF basic/FGF2/bFGF Protein, ≥95% (SDS-PAGE), CAS No.62031-54-3

Synonyms

basic fibroblast growth factor bFGF | Basic fibroblast growth factor | bFGF | FGF basic | FGF2 | FGF-2 | FGFBprostatropin | fibroblast growth factor 2 (basic) | HBGF-2 | heparin-binding growth factor 2 | Prostatropin

Grade

ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance

Specifications & Purity

Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, ≥95%(SDS-PAGE)

Biochemical and Physiological Mechanisms

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and ce

Purity

≥95% (SDS-PAGE)

Bioactivity

Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.

Endotoxin Concentration

<1.0 EU/μg

Expression System

Oryza sativa

Species

Human

Amino Acids

134-288 aa

Sequence

MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

Protein Tag

No tag

Protein Length

Full length protein

Accession #

P09038

Source

Recombinant

Predicted molecular weight

17 kDa

CAS

62031-54-3

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

It is recommended to redissolve the lyophilized powder in sterile water to 100-200 μg/ml, and further dilute it in other solvents.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20 ℃ for 36 months. Upon delivery aliquot. Avoid freeze/thaw cycle. Upon reconstitution, the product should be stored at 2-8 ℃ for 2-7 days and used up as soon as possible. For future use, should be stored at -20 ℃.

Images

Protein Bioactivity: Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp155946)

Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp155946) - Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically less than 1.0 ng/mL.

SDS-PAGE: Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp155946)

Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp155946)-SDS-PAGE
3μg/lane of Recombinant Human FGF2 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.0 kDa.

References

1. Furong Zhang, Kunpeng Jiang, Guisheng Zhu, Huarui Xu, Xiuyun Zhang, Yunyun Zhao, Yejun Zhang, Qiangbin Wang, Pengfei Pang, Aibing Yu.  (2023)  A ZIF-8 composite SiO2-enhanced high-performance PEO-based solid-state electrolyte for Li-metal batteries.  Sustainable Energy & Fuels,  7  (5): (1245-1255).  [PMID:] [10.1039/D2SE01529C]

2. Yang Min, Ma Yuejiang, Cao Zhu, Zhan Hong, Jin Ye, Huang Xiufeng, Yang Shizhou.  (2025)  HPV16 E7 Enhances Cell Stemness via RTKN2-Mediated Activation of the NF-κB Pathway in Cervical Cancer.  BIOCHEMICAL GENETICS,      [PMID:40495058] [10.1007/s10528-025-11144-w]

3. Xiaoshen Zhang, Tao Jiang, Lingyun Ye, Jianguo Sun, Lei Wang, Juanjuan Li, Fengying Wu, Shengxiang Ren, Guanghui Gao.  (2025)  WISP3 upregulates SDCBP expression to promote the progression of non-small cell lung cancer via the TGF-β signaling pathway.  BIOCHEMICAL PHARMACOLOGY,      [PMID:40571215] [10.1016/j.bcp.2025.117082]

4. Jianan Li, Jingqi Huang, Zeyang Gao, Chunpeng He, Zaiyan Xu, Bo Zuo.  (2025)  A novel proliferation synergy factor cocktail maintains proliferation and improves transfection efficiency in muscle cells and fibroblasts under low-serum conditions.  Frontiers in Cell and Developmental Biology,      [PMID:41332986] [10.3389/fcell.2025.1680263]

5. Yang Shizhou, Wu Tingting, Cao Zhu, Chen Zhengyun, Ma Yuejiang, Wang Ting, Qian Linhua, Huang Xiufeng.  (2026)  Hypoxic glycolysis-driven histone lactylation activates NHE7 to promote endometrial cancer progression via COX6C-mediated endoplasmic reticulum stress.  APOPTOSIS,  31  (2): (55).  [PMID:41575611] [10.1007/s10495-026-02262-w]

6. Liping Zhang, Yanyan Wang, Wenxin Qiu, Shuangling Liu, Shengxuan Xu, Hongwei Wang, Lin Yuan.  (2026)  Dual-loaded exosomes with fibroblast growth factor 2 and retinoic acid enhance directed neural differentiation of embryonic stem cells.  COLLOIDS AND SURFACES B-BIOINTERFACES,      [PMID:41691875] [10.1016/j.colsurfb.2026.115554]