Recombinant Human HPRG Protein

Cat. No.: rp147069

CALIDAD & PUREZA Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, His Tag, ≥90%(SDS-PAGE)

Accession #

P04196

Protein Tag

C-10His

Expression system

HEK293

Endotoxin Concentration

<0.1 EU/μg

Bioactivity

Measured by its ability to support the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. When 1 x 10^5 cells/well are added to Recombinant Human HPRG coated plates, adhesion is induced in a dose-dependent manner in the presence of Concanvalin A. The ED50 for this effect is 0.4-2 μg/mL.

Descripción general

Function
Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis.

Especificaciones

Product Name

Recombinant Human HPRG Protein

Sinónimos

Histidine-proline-rich glycoprotein | HPRG | DKFZp779H1622 | Histidine-proline-rich glycoprotein | histidine-rich glycoprotein | HPRG | HRGP | HRG | Thrombophilia due to elevated HRG | THPH11

Grado

ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance, His Tag

Especificaciones y pureza

Animal Free,Carrier Free,Bioactive,ActiBioPure™,High Performance,His-Tag,≥90%(SDS-PAGE)

Bioactividad

Measured by its ability to support the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. When 1 x 10^5 cells/well are added to Recombinant Human HPRG coated plates, adhesion is induced in a dose-dependent manner in the presence of Concanvalin A. The ED50 for this effect is 0.4-2 μg/mL.

Concentración de endotoxinas

<0.1 EU/μg

Sistema de expresión

HEK293

Especie

Human

Aminoácidos

19-525 aa

Secuencia

VSPTDCSAVEPEAEKALDLINKRRRDGYLFQLLRIADAHLDRVENTTVYYLVLDVQESDCSVLSRKYWNDCEPPDSRRPSEIVIGQCKVIATRHSHESQDLRVIDFNCTTSSVSSALANTKDSPVLIDFFEDTERYRKQANKALEKYKEENDDFASFRVDRIERVARVRGGEGTGYFVDFSVRNCPRHHFPRHPNVFGFCRADLFYDVEALDLESPKNLVINCEVFDPQEHENINGVPPHLGHPFHWGGHERSSTTKPPFKPHGSRDHHHPHKPHEHGPPPPPDERDHSHGPPLPQGPPPLLPMSCSSCQHATFGTNGAQRHSHNNNSSDLHPHKHHSHEQHPHGHHPHAHHPHEHDTHRQHPHGHHPHGHHPHGHHPHGHHPHGHHPHCHDFQDYGPCDPPPHNQGHCCHGHGPPPGHLRRRGPGKGPRPFHCRQIGSVYRLPPLRKGEVLPLPEANFPSFPLPHHKHPLKPDNQPFPQSVSESCPGKFKSGFPQVSMFFTHTFPKHHHHHHHHHH

Etiqueta proteínica

C-10His

Número de adhesión

P04196

Peso molecular previsto

59 kDa

SDS-PAGE

65 -75 kDa, under reducing conditions; 55 - 65 kDa, under non-reducing conditions.

Tipo de molécula

Proteína

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Lyophilized

Reconstitución

Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20~-80℃ for more than 1 year. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

SDS-PAGE: Recombinant Human HPRG Protein (rp147069)

Recombinant Human HPRG Protein (rp147069) - SDS-PAGE
2 μg/lane of Recombinant Human HPRG Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 65 -75 kDa under reducing conditions and 55 - 65 kDa under non-reducing conditions.