Recombinant Lambda Light chain Antibody - Anticuerpo primario, specific to Lambda Light chain, Mouse IgG1

Cat. No.: Ab112610

CALIDAD & PUREZA Recombinant, ExactAb™, Validated, 0.02 mg/mL

Application

IHC

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

MyM1-Lambda

Conjugation

Unconjugated

Descripción general

Mouse anti Human Lambda Light chain Antibody, Recombinant (MyM1-Lambda), could be used for IHC and so on.
Application
IHC: 1/100-1/200 
Relevance
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).

Especificaciones

Product Name

Recombinant Lambda Light chain Antibody - Anticuerpo primario, specific to Lambda Light chain, Mouse IgG1

Sinónimos

Bence Jones protein antibody | BJP antibody | Constant region of lambda light chains antibody | Ig lambda chain C regions antibody | ig lambda-6 chain C region antibody | IGLC 1 antibody | IGLC 2 antibody | IGLC 3 antibody | IGLC antibody | IGLC1 antibody

Especificaciones y pureza

ExactAb™, Validated, Recombinant, 0.02 mg/mL

Especies hospedadoras

Ratón

Especificidad

Lambda Light chain

Control positivo

IHC: Human tonsil tissue.

Conjugación

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

0.02 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

Store at 2-8°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

IHC: Recombinant Lambda Light chain Antibody (Ab112610) ​

Recombinant Lambda Light chain Antibody (Ab112610) - IHC
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue labelling Lambda Light chain with Recombinant Lambda Light chain Antibody (Ab112610) at 1/200 dilution. The section was incubated with Ab112610 for 30 mins at room temperature. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was used for antigen retrieval.​

Aplicación

Aplicación

Dilution info

IHC

1/100 - 1/200

Objetivos asociados (humanos)

IGLC1 Tdark Immunoglobulin lambda constant 1 (0 Activities)

Discover Target: IGLC1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

IGLV1-51 Tdark Immunoglobulin lambda variable 1-51 (0 Activities)

Discover Target: IGLV1-51

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Lambda Light chain Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.