Recombinant PHD3 Antibody - Anticuerpo primario, specific to EGLN3, Rabbit IgG

Cat. No.: Ab121571

CALIDAD & PUREZA Recombinant, ExactAb™, Validated, See COA

Application

IF/ICC, IHC, WB

Host species

Conejo

Reactive species

consejo, Humano, Ratón

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human PHD3 Antibody, Recombinant, could be used for WB, IHC and so on.

Especificaciones

Product Name

Recombinant PHD3 Antibody - Anticuerpo primario, specific to EGLN3, Rabbit IgG

Sinónimos

EC 1.14.11 | EC 1.14.11.- | EGL nine (C.elegans) homolog 3 | egl nine homolog 3 (C. elegans) | egl nine homolog 3 | egl nine-like protein 3 isoform | EGLN3 | FLJ21620 | HIF prolyl hydroxylase 3 | HIFPH3 MGC125998 | HIFPH3 | HIF-PH3 | HIF-prolyl hydroxylas

Especificaciones y pureza

Recombinant, ExactAb™, Validated, See COA

Mecanismos bioquímicos y fisiológicos

Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF thro

Especies hospedadoras

Conejo

Especificidad

EGLN3

Inmunógeno

Recombinant protein of human PHD3 (AA 1-239)

Control positivo

WB: Mouse lung, HeLa, A549, MCF7, NIH/3T3 and C6 lysates. IHC: Human colon cancer tissue. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Recombinant PHD3 Antibody (Ab121571)

Recombinant PHD3 Antibody (Ab121571) - Western Blot
All lanes: Recombinant PHD3 Antibody (Ab121571) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 27 kDa
Observed band size: 29 kDa
Exposure time: 58.5 s

IHC: Recombinant PHD3 Antibody (Ab121571)

Recombinant PHD3 Antibody (Ab121571) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant PHD3 Antibody (Ab121571). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Recombinant PHD3 Antibody (Ab121571) - IF/ICC IF analysis of Recombinant PHD3 Antibody (Ab121571) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were st

Recombinant PHD3 Antibody (Ab121571) - IF/ICC
IF analysis of Recombinant PHD3 Antibody (Ab121571) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant PHD3 Antibody (Ab121571) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Aplicación

Aplicación

Dilution info

IHC

1/50 - 1/100

IF/ICC

1/200

WB

1/500-1/1000

Objetivos asociados (humanos)

EGLN3 Tclin Egl nine homolog 3 (0 Activities)

Discover Target: EGLN3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant PHD3 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.