Recombinant Sortase A, CAS No.9033-39-0

CAS: 9033-39-0 Cat. No.: S755808

GRADE & PURITY Recombinant, Endotoxin tested, EnzymoPure™, ≥95%(SDS-PAGE), ≥5.0 mg/mL

Accession #

Q2FV99

Endotoxin Concentration

<5.0 EU/mg

Overview

Sortase A is derived from Staphylococcus aureus and is recombinantly expressed in E. coli. This enzyme is a bacterial transpeptidase that specifically recognizes the LPXTG sequence, cleaves between threonine and glycine, covalently links proteins to the bacterial cell wall, and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell wall peptidoglycan. It can achieve specific conjugation of proteins with polypeptides/chemical groups and has no exogenous protease activity.

Storage solution: 40 mM Tris-HCl, 110 mM NaCl, 2.2 mM KCl, 8 mM imidazole, 0.04% Tween-20, 20% glycerol, pH = 8.0

Transportation and Storage Methods

Storage: This product should be stored at -20°C and can be kept for at least 12 months.

Transportation: Transported with dry ice insulation, with stable activity.

Product Application

This product can site-specifically link proteins/peptides with a C-terminal LPXTG sequence to other proteins or molecules with glycine or aminomethylene groups. It can achieve specific conjugation of proteins with polypeptides/chemical groups and has no exogenous protease activity.

Precautions

After receiving this product, try to avoid repeated freezing and thawing as much as possible;

When using, please wear an experimental coat and disposable gloves;

This product must not be directly used for clinical diagnosis and treatmen.

Specifications

Product Name

Recombinant Sortase A, CAS No.9033-39-0

Synonyms

srtA

Grade

Endotoxin tested, Recombinant, EnzymoPure™

Specifications & Purity

Recombinant, endotoxin tested, EnzymoPure™, ≥95%(SDS-PAGE), ≥5.0 mg/mL

Biochemical and Physiological Mechanisms

Transpeptidase that anchors surface proteins to the cell wall.Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and

Endotoxin Concentration

<5.0 EU/mg

Accession #

Q2FV99

CAS

9033-39-0

Enzyme Commission Number

3.4.22.70

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥5.0 mg/mL

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.