RIC8A Mouse mAb - Anticuerpo primario, specific to RIC8A

Cat. No.: Ab125338

GRADE & PURITY Validated, ExactAb™, See COA

Application

IF/ICC, IHC, WB

Host species

Ratón

Reactive species

consejo, Humano, Ratón

Isotype

Mouse IgG1

Clone Number

OTI3G3

Conjugation

Unconjugated

Descripción general

Mouse anti Human RIC8A Antibody, Monoclonal (OTI3G3), could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

RIC8A Mouse mAb - Anticuerpo primario, specific to RIC8A

Sinónimos

Protein Ric-8A | resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) | RIC8 | RIC8A | RIC8A_HUMAN | synembryn | Synembryn-A | MGC104517 | MGC131931 | MGC148073 | MGC148074 | MGC104517 antibody | MGC131931 antibody | MGC148073 antibody | MG

Especificaciones y pureza

ExactAb™, Validated, See COA

Mecanismos bioquímicos y fisiológicos

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitoti

Especies hospedadoras

Ratón

Especificidad

RIC8A

Inmunógeno

Recombinant full length protein of human RIC8A.

Control positivo

WB: A431, HeLa, K562, U-2 OS, NIH/3T3 and C6 cell lysates. IHC: Human kidney tissue. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY RIC8A, Hep G2 cells.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: RIC8A Mouse mAb (Ab125338)

RIC8A Mouse mAb (Ab125338) - Western Blot
All lanes: RIC8A Mouse mAb (Ab125338) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 65.9 s

IHC: RIC8A Mouse mAb (Ab125338)

RIC8A Mouse mAb (Ab125338) - IHC
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using RIC8A Mouse mAb (Ab125338) (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab125338, Dilution 1/50)

IF/ICC: RIC8A Mouse mAb (Ab125338)

RIC8A Mouse mAb (Ab125338) - IF/ICC
RIC8A Mouse mAb (Ab125338) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RIC8A.

IF/ICC: RIC8A Mouse mAb (Ab125338)

RIC8A Mouse mAb (Ab125338) - IF/ICC
Immunofluorescent staining of Hep G2 cells using RIC8A Mouse mAb (Ab125338).

Aplicación

• Aplicación: Dilution info
• WB:

  1/1000 ;

• IF/ICC:

  1/100;

• IHC:

  1/50

Objetivos asociados (humanos)

RIC8A Tbio Synembryn-A (0 Activities)

Discover Target: RIC8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)