SIL1 Mouse mAb - Anticuerpo primario, specific to SIL1

Cat. No.: Ab127610

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Ratón

Reactive species

consejo, Humano, Ratón

Isotype

Mouse IgG1

Clone Number

OTI3E3

Conjugation

Unconjugated

Descripción general

Mouse anti Human SIL1 Antibody, Monoclonal (OTI3E3), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

SIL1 Mouse mAb - Anticuerpo primario, specific to SIL1

Sinónimos

BAP | BiP associated protein | BiP-associated protein | Endoplasmic reticulum chaperone SIL 1 | Endoplasmic reticulum chaperone SIL1 | MSS | Nucleotide exchange factor SIL 1 | Nucleotide exchange factor SIL1 | SIL 1 | sil1 | SIL1 homolog | SIL1 homolog en

Especificaciones y pureza

ExactAb™, Validated, See COA

Mecanismos bioquímicos y fisiológicos

Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.

Especies hospedadoras

Ratón

Especificidad

SIL1

Inmunógeno

Recombinant protein of human SIL1.

Control positivo

WB: Hep G2, HeLa, A549 and A431 cell lysates. IHC: Human kidney, liver, carcinoma of liver, adenocarcinoma of ovary tissues. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY SIL1, HT29 cells. Flow Cytometry: HEK293T transfected with pCMV6-ENTRY SIL1.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - Western Blot
All lanes: SIL1 Mouse mAb (Ab127610) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 48, 54 kDa
The expression profile and molecular weight observed, which is due to glycosylation.
Exposure time: 57.2 s

IHC: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using SIL1 Mouse mAb (Ab127610). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab127610, Dilution 1/50)

IHC: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using SIL1 Mouse mAb (Ab127610). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab127610, Dilution 1/50)

IHC: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using SIL1 Mouse mAb (Ab127610). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab127610, Dilution 1/50)

IHC: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using SIL1 Mouse mAb (Ab127610). (Heatinduced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab127610, Dilution 1/50)

IF/ICC: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - IF/ICC
SIL1 Mouse mAb (Ab127610) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIL1.

IF/ICC: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - IF/ICC
Immunofluorescent staining of HT29 cells using SIL1 Mouse mAb (Ab127610).

Flow Cytometry: SIL1 Mouse mAb (Ab127610)

SIL1 Mouse mAb (Ab127610) - Flow Cytometry
HEK293T cells transfected with either pCMV6-ENTRY SIL1 (Red) or empty vector control plasmid (Blue) were immunostained with SIL1 Mouse mAb (Ab127610), and then analyzed by flow cytometry.

Aplicación

• Aplicación: Dilution info
• IHC:

  1/100-1/500;

• IF/ICC:

  1/100;

• WB:

  1/200-1/1000;

• Flow Cytometry:

  1mg/ml;

Objetivos asociados (humanos)

SIL1 Tbio Nucleotide exchange factor SIL1 (0 Activities)

Discover Target: SIL1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)