SNET Lysis Buffer (pH 8.0, Sterile) - BioReagent

Cat. No.: S1528863

GRADE & PURITY BioReagent

Storage

Store at 2-8°C

Shipped In

Wet ice

Descripción general

This product is composed of Tris-Cl (pH 8.0), EDTA (pH 8.0), NaCl, SDS, and other components. It is sterilized by filtration and is intended for the lysis of animal tissue samples for DNA extraction. It effectively facilitates the release of nucleic acids while inhibiting nuclease activity. For research use only.

Specifications

Especificaciones y pureza

BioReagent

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent

Product Line

Productos biológicos

Application Protocols

Item-specific facts (from Product Data)

• Ready-to-use sterile lysis buffer at pH 8.0. No validated, product-specific protocols are provided in the listing; refer to your method SOPs and the CoA/Spec Sheet.

Example workflows (literature/general knowledge; verify and optimize for your system)

1. Mammalian cell lysis for DNA prep

• Pellet 1×10^6–1×10^7 cells; remove supernatant.
• Add 0.2–1.0 mL of lysis buffer; pipette gently to resuspend.
• Incubate 10 minutes at room temperature or on ice, gently inverting.
• Optional: Add RNase A for DNA-only prep; incubate 10 minutes at 37°C. Clarify by centrifugation and proceed to alcohol precipitation or column cleanup.

2. Tissue lysis for protein analysis

• Weigh tissue; add 10 volumes buffer (w/v). Homogenize on ice (Dounce or rotor–stator).
• Incubate 15 minutes on ice with occasional mixing. Clarify at 12,000–20,000 × g for 15 minutes at 4°C.
• Determine protein concentration (a detergent-compatible assay if detergent is present in your lysis matrix). Proceed to SDS-PAGE after buffer exchange/detergent removal if necessary.

3. Crude RNA extraction (detergent-based)

• Lyse cells/tissue as above, then add equal volume of phenol-free extraction solution only if validated for your matrix; otherwise, proceed directly to silica column after adjusting binding conditions per kit instructions.

Notes

• Detergent and chelator content for this item are not specified; ensure downstream assay compatibility and include appropriate controls.

Biological Roles

Applicability

• As a formulated laboratory buffer, the product itself does not have intrinsic biological roles in organisms. It is used to manipulate biological samples in vitro.

Literature/general knowledge on typical components and rationale

• Buffering near pH 8.0: Many macromolecules, including DNA, are more stable near neutral to slightly basic pH. Tris-based systems (literature) are commonly chosen due to pKa ~8.06 at 25°C and effective buffering range around pH 7–9.
• Chelators (e.g., EDTA; literature) are often included in lysis buffers to sequester divalent cations (Mg2+, Ca2+), inhibiting DNases and some metalloproteases, thereby preserving nucleic acids and proteins.
• Detergents: Nonionic or ionic detergents (literature) disrupt lipid bilayers and can unfold proteins, aiding solubilization. Choice of detergent dictates preservation of complexes vs denaturation.
• Salt: Sodium chloride or similar salts modulate ionic strength, influencing protein solubility, nucleic acid precipitation thresholds, and DNA-protein interactions.

Use-context guidance

• For genomic DNA extraction, gentle mixing and chelation at pH 8 can maintain high molecular weight DNA. For proteomics, detergent removal and buffer exchange are typically required before digestion or LC-MS.

Item-specific disclaimer

• The exact composition and any enzyme inhibitor content of this catalog item are not specified. Users should consult the CoA/SDS and optimize protocols based on their analyte and downstream application.

Buffer Applications

Item-specific facts (from Product Data)

• SNET Lysis Buffer (pH 8.0, Sterile); ready-to-use lysis buffer for research use only. Additional formulation details are not specified for this item; refer to CoA/Spec Sheet.

General buffer application guidance (literature/general knowledge)

• pH 8.0 is widely used for nucleic-acid and protein lysis/extraction workflows. It balances nucleic acid stability with moderated protease/nuclease activity.
• Tris-based systems (literature) are commonly used around pH 8 due to pKa ~8.06 (25°C) and a temperature coefficient of ~−0.028 pH/°C. If calibrating or verifying pH, measure at the intended use temperature.
• Typical workflows: Cell culture lysis, tissue homogenization, crude lysate preparation before DNA/RNA extraction or protein purification, and pre-clear steps before immunoprecipitation or chromatography.

Practical usage notes

• Volumes: A common starting point is 5–10 volumes of buffer per wet tissue weight (w/v) or sufficient buffer to fully wet cell pellets (e.g., 5–10 mL per 1×10^8 cells), then adjust empirically based on viscosity and target concentration (literature guidance).
• Mixing: Use gentle pipetting or inversion for nucleic acids; combine with mechanical disruption for tough tissues.
• Clarification: Centrifuge to remove debris; retain supernatant for downstream processing. For nucleic acids, follow with precipitation or column purification; for proteins, consider desalting/detergent removal before analysis.

Compatibility

• Detergent and salt content (if present) can interfere with electrophoresis, enzyme assays, or MS. Plan cleanup steps accordingly.

Note

• Because formulation is not specified for this item, validate buffer compatibility with your method and controls.

Green Alternatives

Context

• This product is an aqueous lysis buffer; environmental considerations primarily involve detergent/chelating content, salts, and biological load from lysates rather than volatile organic solvents.

Greener practice considerations (literature/general knowledge)

• Prefer aqueous, room-temperature lysis where feasible to minimize energy usage. This buffer’s pH 8.0 supports many ambient workflows.
• If your process is detergent-intensive, evaluate milder or biodegradable surfactants and minimize concentrations consistent with performance. Some nonionic detergents are less problematic for wastewater treatment than ionic surfactants.
• Reduce hazardous chaotropes: Where possible, favor detergent/salt-based aqueous lysis over guanidinium thiocyanate or phenol-based extraction to lessen hazardous waste streams, provided your targets and yields are acceptable.
• Waste management: Segregate biological waste (post-lysis) from chemical waste. Neutralize pH if required by local regulations and avoid discharge of strong chelators that can mobilize heavy metals in plumbing; collect for proper disposal if chelators are present.

Comparison examples (illustrative; not item-specific)

• Aqueous lysis buffer (this category) vs phenol–chloroform: Aqueous avoids halogenated solvent waste and reduces exposure risks, but may require additional cleanup for nucleic acids.
• Detergent-based lysis vs chaotrope-based lysis: Detergent-based approaches are often safer to handle and produce less hazardous waste, but may challenge downstream MS and some chromatography unless removed.

Note

• Composition for this item is not specified; verify your environmental health and safety (EHS) plan against the actual SDS.

Pharmaceutical Uses

Scope and limitations

• This product is labeled For research use only and is not intended for human or veterinary use, diagnostic procedures, or as a drug/excipient in commercial manufacturing.

Literature/general knowledge

• Lysis buffers are used in bioprocess development labs for sample preparation, analytics, and method development surrounding upstream and downstream processes (e.g., titer assays, impurity profiling). They can support feasibility studies for cell disruption parameters and extraction efficiencies.
• In regulated environments, buffers employed in GMP manufacturing or QC must meet compendial/validated specifications (e.g., USP/EP grades, documented bioburden/endotoxin, particulate, and container-closure integrity). Those attributes are not specified for this item.

Practical guidance

• For translational or manufacturing applications, transition from research-grade lysis buffers to qualified, documented formulations with traceable raw materials and validated sterilization. Define acceptance criteria for pH, conductivity, bioburden/endotoxin, and particulate.
• Document change control when bridging from research-grade materials to GMP-grade equivalents; requalify key analytical methods due to potential matrix effects from detergent/salt differences.

Item-specific note

• No pharmacopeial status, endotoxin limits, or residual solvent/detergent specifications are provided for this product. Consult CoA/Spec Sheet if pursuing method development activities adjacent to regulated workflows.

Physical Properties

Item-specific facts (from Product Data)

• Physical state: Aqueous buffer solution, sterile (appearance not specified for this item; refer to CoA/Spec Sheet.)
• pH: 8.0 (at formulation; temperature dependence not specified for this item.)
• Storage: 2–8°C (see Storage & Reconstitution tab for handling.)
• Other properties (density, osmolality/ionic strength, conductivity, refractive index, viscosity, UV cutoff): Not specified for this item; refer to CoA/Spec Sheet.

Literature/general values (for similar SNET-type buffers; not item-specific)

• Typical pH control near 8.0 is often achieved with Tris-based buffers; Tris has pKa ~8.06 at 25°C (literature). Buffer effective range ~pH 7–9. Temperature coefficient for Tris is approximately −0.028 pH/°C (literature), meaning measured pH will vary with temperature.
• Aqueous lysis buffers are fully miscible with water and generally immiscible with hydrophobic solvents. Conductivity typically scales with salt content; for example, 100–200 mM NaCl solutions exhibit conductivities on the order of several to tens of mS/cm (literature), but this product’s conductivity is not specified.

Practical notes

• Because exact ionic strength and detergent content are not specified for this item, users running conductivity- or detergent-sensitive downstream assays (e.g., mass spectrometry, electrophoresis) should perform a small-scale compatibility check and, if needed, dialyze, desalt, or precipitate analytes prior to analysis.

Quality and Grades

Item-specific facts (from Product Data)

• Grade/Purity: BioReagent.
• Sterility: Sterile (item-specific).
• Other quality attributes (endotoxin level, DNase/RNase/protease free status, particulate/bioburden limits, UV absorbance profile): Not specified for this item; refer to CoA/Spec Sheet.

What BioReagent grade generally implies (literature/general knowledge)

• BioReagent-grade materials are suitable for common laboratory biological workflows where consistent performance, low impurity background, and reproducibility are required. For buffers, this typically means controlled raw material quality and manufacturing under procedures designed to limit bioburden and particulate matter.
• Sterile designation indicates the final solution has been sterile-filtered and/or aseptically processed to meet a defined sterility assurance level. It is appropriate for workflows where microbial contamination would compromise results (e.g., culture-adjacent lysis, nucleic-acid and protein preparations).

Practical guidance

• For nuclease- or protease-critical workflows, verify whether the lot is qualified as DNase/RNase/protease-free on the CoA if your method requires it; this is not specified for this item.
• If low background UV absorbance for spectrophotometry or LC/UV is required, confirm the UV profile on the CoA/Spec Sheet; not specified here.
• Record lot numbers, and, for regulated labs, retain CoA and sterility documentation with your batch records.

Reaction and Applications

Item-specific facts (from Product Data)

• Intended use: Lysis buffer for laboratory research workflows; pH 8.0; sterile. For research use only.

Applications and rationale (literature/general knowledge)

• DNA and RNA preparation: pH 8 buffers help maintain nucleic acid integrity during extraction by minimizing acid-catalyzed hydrolysis and depurination. Chelators (if present in a given SNET-style buffer) can sequester Mg2+ to inhibit nucleases, while detergents disrupt lipid bilayers to release nucleic acids.
• Protein extraction: Lysis buffers solubilize proteins from cells/tissues. Depending on detergent type and salt content, they can preserve native complexes or enforce denaturing conditions. pH 8 aligns with many enzyme compatibility windows for downstream assays after buffer exchange.
• Tissue homogenization: When combined with mechanical disruption (bead beating, rotor–stator, sonication), lysis buffers facilitate efficient release of intracellular components.
• Sample cleanup workflows: Post-lysis, common steps include clarification by centrifugation, nuclease/protease inhibition (if not already built-in), and buffer exchange via desalting or precipitation depending on the target biomolecule.

Practical tips

• Keep samples cold (on ice or 2–8°C) during lysis to reduce proteolysis and nuclease activity. Prechill buffer and equipment.
• Detergent content (if any) can interfere with downstream electrophoresis or MS; plan for removal (e.g., acetone/TCA protein precipitation, SPE, phase-transfer surfactants) or select compatible methods.
• For nucleic acids, avoid vigorous vortexing after lysis to reduce shearing if high-molecular-weight DNA is required. For RNA work, ensure RNase-free practice and consumables.

Note

• Specific composition and inhibitor content for this item are not specified; adjust protocols accordingly.

Reaction Conditions

Applicability

• This section provides general, literature-based guidance on lysis conditions using pH ~8 buffers. It does not specify the exact operating conditions for this item’s formulation.

General lysis conditions (literature/general knowledge)

• Temperature: Keep on ice or 2–8°C for proteome and nucleic-acid integrity. Some workflows incubate at 25°C for 5–15 minutes to facilitate detergent action; DNA extraction from tough tissues may benefit from 37–56°C incubations when proteinase K is added (if compatible).
• Time: 5–30 minutes for cultured cells; 15–60 minutes with intermittent mixing for tissues. Monitor viscosity (DNA release) as an indicator of lysis.
• Ratios: Start with 5–10 volumes of buffer per unit wet tissue mass or sufficient buffer to resuspend cell pellets thoroughly. Adjust based on target concentration and viscosity.
• Agitation: Gentle inversion for nucleic acids; mechanical disruption (bead mill, Dounce, rotor–stator) for fibrous tissues. Avoid excessive shear if high-molecular-weight DNA is desired.
• Clarification: Centrifuge 10,000–20,000 × g, 10–20 minutes at 4°C to remove debris (parameters vary by sample type and tube rating).

Downstream considerations

• Detergents and salts may inhibit enzymes (PCR, restriction digestion) and interfere with MS or isoelectric focusing. Employ cleanup: ethanol/IPA precipitation for nucleic acids, protein precipitation or detergent-removal spin columns for proteomics.

Item-specific note

• This product’s exact detergent, salt, and additive profile is not specified. Optimize conditions empirically and consult the SDS/CoA for constraints.

Safety and Handling

Item-specific facts (from Product Data)

• GHS classification, signal word, pictograms, and H-statements: Not specified for this item; refer to the product SDS.
• Storage conditions: Store at 2–8°C. Shipped on wet ice.

General safety guidance (literature/general knowledge)

• Lysis buffers may contain detergents and chelators that can be irritants to skin/eyes and harmful if swallowed. Handle with appropriate PPE: lab coat, nitrile gloves, and safety glasses or face shield.
• Avoid inhalation of aerosols and contact with skin/eyes. Work in a clean area to prevent contamination of samples and exposure to biohazards from lysed materials.
• Incompatibilities: Do not mix with strong oxidizers or acids/bases unless process-validated. Certain detergents can foam excessively; add slowly when combining with other solutions. Chelators (if present) can sequester metal ions; avoid contact with metal-sensitive assays or chromatography resins without validation.
• First aid (overview; defer to SDS): In case of skin/eye contact, rinse with water for at least 15 minutes; remove contaminated clothing. If inhaled, move to fresh air. If ingested, rinse mouth; seek medical attention. Provide SDS to medical personnel.
• Spill response: Absorb with inert material (e.g., paper towels, spill pads), collect in compatible container, and wash area with water. Dispose in accordance with institutional and local regulations.

Note

• Always consult the product SDS for authoritative hazard, exposure limits, and response procedures.

Solvent Selection

Applicability

• This product is itself an aqueous buffer solution and is not used as a solvent selection case in organic synthesis. However, solvent compatibility with downstream steps is important in biochemistry workflows.

General solvent and miscibility considerations (literature/general knowledge)

• Polarity: Highly polar, water-based; miscible with water and aqueous buffers. Typically immiscible with nonpolar organic solvents unless emulsified by detergents.
• Dielectric environment: High dielectric constant (that of water at 25°C ~78.4; literature). Ionic strength and presence of detergents (if any) can alter macromolecular interactions.
• Compatibility: Suitable for lysis of cells and tissues before nucleic-acid or protein extraction. For downstream organic extractions (e.g., phenol–chloroform), detergent and salt content can affect phase separation; preclear or adjust composition as needed.

Selection tips

• Choose this buffer when pH 8.0 lysis conditions are desired to stabilize nucleic acids and minimize acid-mediated depurination while keeping many enzymes (e.g., nucleases) less active.
• If your downstream application is mass spectrometry, assess detergent removal methods (e.g., protein precipitation, SPE, FASP) to mitigate ion suppression.
• For chromatography or electrophoresis, match ionic strength and pH to method requirements; desalting (spin columns, dialysis) may be required if salt concentration is high.

Alternatives

• For detergent-free lysis, consider mechanical disruption in a simple pH 8 buffer (literature). For stronger denaturation, chaotrope-based solutions (e.g., guanidinium) can be used, balancing safety and waste considerations.

Storage and Reconstitution

Item-specific facts (from Product Data)

• Storage conditions: Store at 2–8°C.
• Shipping: Shipped on wet ice.
• Format: Sterile aqueous solution; ready-to-use. Reconstitution is not required.
• Additional stability details (shelf life, freeze–thaw tolerance, light sensitivity, DNase/RNase/protease-free status, preservative content): Not specified for this item; refer to CoA/Spec Sheet.

General handling guidance (literature/general knowledge)

• Avoid repeated freeze–thaw cycles of buffer stocks; if freezing is necessary for long-term storage and the composition permits, aliquot into sterile containers to minimize cycles. Note that some detergents can precipitate or phase-separate upon cold storage; warm to room temperature and mix gently to re-dissolve.
• Maintain sterility: Open in a clean environment. Use sterile pipette tips and vessels. Do not top off bottles or return used portions to the stock container.
• Pre-use: Equilibrate to intended working temperature; verify pH at that temperature if critical to your assay, as pH can shift with temperature (notably for Tris-based systems; literature).
• Visual inspection: Before each use, inspect for turbidity, precipitate, or container compromise. Do not use if contamination or precipitation is observed without confirming compatibility.

Disposal

• Dispose of spent buffer and lysate waste according to institutional biosafety and chemical hygiene plans. Deactivate biological materials as required before disposal.

Structure and Identity

Item-specific facts (from Product Data)

• Product type: Sterile, ready-to-use lysis buffer (aqueous solution), pH 8.0
• Grade: BioReagent
• Single-molecule identifiers (CAS, CID, InChIKey, SMILES, molecular formula, molecular weight): Not applicable to a formulated mixture; not specified for this item; refer to CoA/Spec Sheet.

General description (literature/general knowledge)

• SNET-style lysis buffers are commonly used for disrupting cells and tissues and stabilizing nucleic acids and proteins during extraction. They typically contain a buffering component near pH 8, salts for ionic strength, and often chelators and detergents to aid lysis and inhibit nuclease activity. Exact composition varies by supplier and application.
• Structural features: As a mixture, this product does not have a single defined 2D/3D structure. Typical constituents (literature) may include a primary amine buffer (e.g., Tris base/Tris-HCl), sodium salts (e.g., NaCl), chelators (e.g., EDTA), and a surfactant. These components present ionic and polar functional groups (quaternary/primary ammonium, carboxylate/chelating oxygens, sulfate/ether for certain detergents), conferring high aqueous solubility and protein/nucleic-acid interaction capability.
• Stereochemistry: Not applicable to the mixture as a whole.

Notes

• Do not infer composition or concentrations from the name alone; for this catalog item, specific ingredients and their levels are not specified. Always consult the SDS and CoA for definitive identity and regulatory documentation.

Synthetic Utility

Not typically applicable

• As a biological lysis buffer, this product is not used as a reagent or solvent in small-molecule synthetic chemistry. It does not provide defined functional groups for transformations, nor does it serve as a catalyst or coupling partner.

Related practical insights (literature/general knowledge)

• In chemical biology or bioconjugation workflows, lysates prepared with lysis buffers can be sources of biomolecules (proteins, nucleic acids, metabolites) for subsequent labeling or analysis after buffer exchange. Detergents and chelators, if present, must be removed or neutralized to avoid interfering with coupling chemistries (e.g., NHS-ester labeling, click reactions, metal-catalyzed conjugations).
• For enzyme-mediated transformations studied in vitro, lysis buffer components (pH, ionic strength, chelators) can strongly affect enzymatic rates and metal-dependent enzymes. Prior to kinetic or mechanistic studies, exchange lysate into a defined assay buffer to ensure reproducibility.

Item-specific disclaimer

• Composition (salt, detergent, chelator, additive content) is not specified for this item; verify compatibility with your chemistry or bioconjugation step and perform small-scale tests.

Target Specificity

Not applicable to this product

• This item is a general-purpose lysis buffer and does not possess target specificity in the sense of antibodies, probes, or inhibitors. No antigen, epitope, clone, isotype, or species reactivity information is relevant.

Guidance

• If selective lysis is desired (e.g., differential lysis of erythrocytes vs leukocytes), that depends on buffer composition and conditions. Since this item’s detailed formulation is not specified, users should validate selectivity empirically or use a formulated selective lysis buffer designed for that application.