T4 Lysozyme

Cat. No.: T1509550

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), 1 mg/ml

Accession #

P00720

Expression system

E. coli

Bioactivity

A 200 μl reaction in 50 mM Tris-HCl pH 8.0 buffer containing a Micrococcus lysodeikticus suspension and 1 μg of T4 Lysozyme (E. coli) incubated for 20 minutes at 25°C results in a decrease of ≥0.05 in turbidity as determined by OD₄₅₀.

Overview

Bacteriophage T4 Lysozyme breaks down bacterial cell walls. The enzyme attacks the peptidoglycans in the cell walls of bacteria and hydrolyzes the ß-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine. Specific activity of T4 lysozyme is significantly greater than egg white lysozyme when assayed with Micrococcus lysodeikticus and E. coli.

Protein Purity: ≥95% (SDS-PAGE) 

Storage Buffer: 20 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 0.1mM EDTA, 50% Glycerol, pH 8.0.

Activity: 200 μl reaction in 50 mM Tris-HCl pH 8.0 buffer containing a Micrococcus lysodeikticussuspension and 1μg of T4 Lysozyme (E. coli) incubated for 20 minutes at 25°C results in a decrease of ≥0.05 in turbidity as determined by OD₄₅₀.  

Applications:

Bacterial lysis for nucleic acid extraction

Bacterial lysis for recombinant protein extraction

Source:

A recombinant E. coli strain carrying the cloned bacteriophage T4 lysozyme gene.

Specifications

Product Name

T4 Lysozyme

Synonyms

muramidase | Endolysin

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), 1 mg/ml

Biochemical and Physiological Mechanisms

Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabil

Bioactivity

A 200 μl reaction in 50 mM Tris-HCl pH 8.0 buffer containing a Micrococcus lysodeikticus suspension and 1 μg of T4 Lysozyme (E. coli) incubated for 20 minutes at 25°C results in a decrease of ≥0.05 in turbidity as determined by OD₄₅₀.

Accession #

P00720

Enzyme Commission Number

EC 3.2.1.17

Molecule Type

Enzyme

Storage and Shipping

Concentration

1 mg/ml

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.