TMC2 Human Pre-designed siRNA Set A

Cat. No.: T1470135

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Descripción general

TMC2 Human Pre-designed siRNA Set A contains three designed siRNAs for TMC2 gene (Human), as well as a negative control, a positive control, and a FAM-labeled negative control. Components TMC2 siRNA-1: 5 nmol (HPLC) TMC2 siRNA-2: 5 nmol (HPLC) TMC2 siRNA-3: 5 nmol (HPLC) siRNA Negative Control: 5 nmol (HPLC) FAM-labeled siRNA Negative Control: 5 nmol (HPLC) GAPDH siRNA Positive Control:5 nmol (HPLC)

Specifications

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Application Protocols

• Item-specific

  • Tested applications, recommended working concentrations, and detailed protocols are not specified for this item; refer to CoA/Spec Sheet and SDS.

• Note

  • As only product-data-sourced protocols are to be listed here, no additional procedures are provided.

Biological Roles

• Item-specific (Product Data)

  • Target: Human TMC2 (Transmembrane Channel-Like 2), per product name. No additional item-specific biological details provided.

• General/literature overview of TMC2 biology

  • Family/context: TMC2 is a member of the transmembrane channel-like (TMC) family. TMC1 and TMC2 have been implicated in the mechanotransduction apparatus of inner ear hair cells in vertebrates.
  • Function: Evidence suggests TMC proteins contribute to, or form part of, the mechanically gated transduction channel complex in hair cells. TMC2 expression is enriched in developing auditory/vestibular tissues and may complement or partially compensate for TMC1 in certain contexts.
  • Cellular localization: Predicted multi-pass membrane protein localized to stereocilia/hair bundle regions (literature).
  • Pathway relevance: Studies using siRNA/CRISPR perturbations and genetic models explore TMC2 roles in mechanosensitive ion flux, hair cell development, and maintenance. These insights support basic research into sensory transduction mechanisms.

• Research implications

  • Silencing TMC2 with siRNA enables investigators to probe gene-specific contributions to mechanotransduction phenotypes, dissect redundancy with TMC1, and analyze downstream transcriptional responses.

• Note

  • The above biological roles are literature-based and provided for research context. They are not product specifications and do not imply suitability for clinical or diagnostic use.

Buffer Applications

• Applicability

  • As a nucleic-acid reagent, this product is used in aqueous, RNase-free buffers rather than classical small-molecule buffer systems.

• General/literature practices

  • Reconstitution: Common choices include RNase-free water or TE buffer (e.g., 10 mM Tris-HCl, pH 7.5–8.0; 0.1–1 mM EDTA) to improve storage stability. Not specified for this item.
  • Working solutions: Dilute into serum-free medium for complex formation with transfection reagent; then add to cells with or without serum depending on reagent guidance. Maintain isotonic, physiological pH (7.2–7.4).
  • RNase control: Use DEPC-treated or nuclease-free certified solutions and consumables. Avoid Mg2+/Ca2+-rich buffers unless required, as divalent cations can support nuclease activity.

• Not item-specific

  • No buffer recipes or concentrations are specified for this item; refer to CoA/Spec Sheet and the chosen transfection reagent’s manual for exact buffer recommendations.

Green Alternatives

• Context

  • siRNA experiments are inherently aqueous and typically free of large volumes of hazardous organic solvents. Environmental considerations focus on cold-chain logistics, single-use plastics, and decontamination chemicals.

• Greener practice suggestions (general)

  • Minimize cold shipments: Consolidate orders to reduce packaging waste and shipping frequency. Store at -20°C to maintain stability and avoid reshipments.
  • Buffer selection: Use minimal, non-hazardous aqueous buffers (RNase-free water or low-EDTA TE). Avoid unnecessary antimicrobial additives.
  • Plastic reduction: Employ reusable RNase-free racks and choose low-binding consumables judiciously to reduce waste.
  • Decontamination: Prefer RNase-inactivating solutions with lower VOC content; avoid chlorine-based agents when not required.

• Trade-offs

  • Stringent RNase control often necessitates single-use certified consumables, increasing plastic waste. Balance sustainability with data integrity by batching experiments and right-sizing reaction volumes.

• Item-specific note

  • No alternative formulations or stabilization chemistries are specified for this item; consult CoA/Spec Sheet if green chemistry attributes (e.g., minimized hazardous residuals) are provided.

Pharmaceutical Uses

• Item-specific (Product Data)

  • Research Use Only: For research use only; not for human or veterinary use. No GMP or pharmacopeial status specified.

• General context (non-clinical)

  • In pharmaceutical discovery labs, pre-designed siRNA sets are used for target validation, pathway interrogation, and phenotypic screening in cell-based assays. Data support decision-making in early discovery but are not themselves clinical materials.
  • Formulation aspects for in vitro work include complexation with cationic lipids or polymers to enhance cell uptake; these conditions are assay-specific and not specified for this item.

• Compliance note

  • This product is not intended for therapeutic, diagnostic, or ex vivo clinical manufacturing. Consult institutional policies for handling nucleic-acid research reagents.

Physical Properties

• Item-specific (Product Data)

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

• General/literature values for siRNA oligonucleotides

  • Length: Typically ~21–23 nucleotides per strand (literature).
  • Approx. molecular mass: ~13–15 kDa per duplex for 21-mers (varies with sequence and modifications; literature). Not a specification for this item.
  • Solubility: Highly soluble in RNase-free water and low-salt aqueous buffers (e.g., 10 mM Tris, pH ~7.5–8.0) (literature).
  • UV absorbance: Strong absorbance at 260 nm; extinction coefficient depends on sequence and length (literature). Not specified for this item.
  • pKa/logP: Not typically defined for polymers/biopolymers; siRNA is a polyanion with multiple phosphate groups (literature).
  • Thermal behavior: Duplex melting temperature (Tm) commonly in the 50–80°C range depending on GC content and length (literature). Not specified for this item.

• Practical notes (general)

  • Avoid repeated freeze–thaw cycles, which can promote strand degradation and adsorption losses.
  • Use low-binding plastics and RNase-free conditions to preserve integrity and concentration.

Quality and Grades

• Item-specific (Product Data)

  • Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.

• Interpreting typical siRNA quality descriptors (general/literature)

  • Purification: siRNA duplexes are commonly supplied desalted, PAGE-purified, or HPLC-purified. Higher purity (PAGE/HPLC) reduces truncated species and can improve knockdown consistency, especially for sensitive applications. The exact purification for this item is not specified.
  • Identity/Content: Vendors often provide OD260 or nmol content, and sometimes mass spectrometry identity confirmation. For this item, specific metrics are not specified.
  • End modifications: Some designs include 5′-phosphate, 3′-dTdT overhangs, or chemical stabilizations (e.g., minimal 2′-OMe). Such features influence nuclease resistance and innate immune stimulation; not specified for this item.
  • Design quality: Pre-designed sets typically target multiple regions of the transcript and are screened in silico for off-target minimization (e.g., seed-match filtering, SNP avoidance). Details not specified for this item.

• Practical recommendations

  • Consult the product CoA/Spec Sheet for the exact purification method, yield, and any modifications.
  • When comparing with other sources, align on purification level and strand phosphorylation state to ensure experimental comparability.

Reaction and Applications

• Item-specific (Product Data)

  • Intended use category: Research use only.
  • Application theme: Gene-expression knockdown of human TMC2 via RNA interference (implied by product name). No further item-specific application details provided.

• General/literature applications of pre-designed siRNA sets

  • RNAi-mediated silencing: Duplex siRNA is incorporated into RISC; the antisense strand guides sequence-specific endonucleolytic cleavage of target mRNA, reducing protein expression.
  • Use cases: Pathway analysis, target validation, phenotypic screening, and mechanistic studies. Sets of multiple siRNAs improve confidence via concordant phenotypes and allow follow-up with the best-performing sequence.
  • Controls: Include non-targeting siRNA and, where possible, a positive control siRNA to validate transfection efficiency and assay sensitivity.
  • Readouts: mRNA knockdown quantified by RT-qPCR (24–48 h), protein reduction by immunoblotting or immunostaining (48–96 h), and functional assays aligned to TMC2 biology (e.g., ion-channel or mechanotransduction proxies in relevant cell models).

• Practical tips (general)

  • Start with a small dose–response (e.g., 1–50 nM final) to identify minimal effective concentration that achieves robust knockdown with low cytotoxicity.
  • Optimize transfection reagent, complexation time, and cell density for each cell line. Verify knockdown kinetics before endpoint assays.

Reaction Conditions

• Not typically applicable

  • Classical reaction conditions (solvent, catalysts, temperature profiles) do not apply to this siRNA product.

• General/literature note for cellular use

  • For cell transfection, conditions (cell density, siRNA concentration, complexation time, medium composition) are determined by the chosen transfection reagent and cell line. These are not specified for this item; consult your transfection reagent’s instructions and optimize empirically.

Safety and Handling

• Item-specific (Product Data)

  • GHS classification, signal word, H-statements, pictograms: Not specified for this item; refer to SDS.
  • Storage conditions: Store at -20°C.
  • Shipping: Ice chest + ice pads (cold-chain).

• General safety guidance for siRNA oligonucleotides (literature/practice)

  • Hazard profile: Synthetic short RNAs are generally considered of low acute toxicity; however, handle as a laboratory chemical/biological reagent. Do not ingest, inhale, or allow skin/eye contact.
  • PPE: Laboratory coat, nitrile gloves, and safety glasses recommended. Work in a clean area to minimize RNase contamination.
  • Incompatibilities: RNases present on skin, common lab surfaces, and non-sterile consumables rapidly degrade RNA. Use RNase-free water/buffers, certified RNase-free tubes and tips, and clean benches with RNase-decontamination solutions.
  • First aid (overview; defer to SDS): In case of contact, rinse skin/eyes with water. If inhaled, move to fresh air. If ingested, rinse mouth and seek medical attention if unwell.
  • Waste: Dispose of small quantities in accordance with institutional guidelines for non-hazardous biological/chemical waste unless local rules specify otherwise.

• Stability considerations (general)

  • Protect from repeated freeze–thaw cycles and prolonged exposure to elevated temperatures. Minimize exposure to nucleases by maintaining aseptic, RNase-free technique.

Solvent Selection

• Applicability to siRNA

  • siRNA reagents are aqueous-biopolymer materials, not small-molecule organics. Solvent selection centers on RNase-free aqueous buffers rather than organic solvent systems.

• General/literature guidance

  • Primary solvents: RNase-free water or TE buffer (10 mM Tris-HCl, 0.1–1 mM EDTA, pH 7.5–8.0) are commonly used for stock solutions. EDTA chelates trace divalent cations that may promote nuclease activity.
  • Compatibility: Stocks prepared in water/TE are typically diluted into serum-containing or serum-free cell culture media for transfection, in the presence of a lipid/polymer transfection reagent. Avoid strong acids/bases and organic solvents that can denature nucleic acids and harm cells.
  • Salt considerations: Low ionic strength buffers reduce aggregation; physiological buffers (e.g., PBS) are acceptable for short handling but may not be optimal for long-term storage due to divalent cation content.

• Comparison (general)

  • Water vs TE: Water minimizes additives but offers less nuclease protection; TE can enhance stability during storage. Choice depends on duration of storage and downstream use. For immediate transfection, RNase-free water stocks are commonly used to simplify complex formation.

• Item-specific note

  • No reconstitution buffer is specified for this item; refer to CoA/Spec Sheet for the recommended solvent.

Storage and Reconstitution

• Item-specific (Product Data)

  • Storage: Store at -20°C.
  • Shipping: Ice chest + Ice pads (maintain cold-chain).
  • Reconstitution buffer, concentration, and aliquoting guidance: Not specified for this item; refer to CoA/Spec Sheet.

• General caution

  • Avoid repeated freeze–thaw cycles; prepare single-use aliquots upon first thaw if recommended by the CoA. Use RNase-free consumables to prevent degradation.

Structure and Identity

• Item-specific (Product Data)

  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey / SMILES: Not applicable for siRNA oligonucleotide mixtures; not specified for this item.
  • Product type: Pre-designed siRNA Set A targeting human TMC2 (per product name).

• General/literature description for pre-designed siRNA sets

  • Composition: Typically a set of 2–4 double-stranded RNA duplexes (each ~21–23 nt) with 2-nt 3′ overhangs (e.g., dTdT) designed to target distinct regions of the same mRNA to increase knockdown reliability.
  • Structural features: Antiparallel, partially duplexed RNA with ribose 2′-OH groups; often sense/antisense strands are phosphorylated at 5′ ends (literature). May include chemical stabilizations (e.g., minimal 2′-OMe at select positions) depending on supplier; not specified for this item.
  • Target locus: TMC2 gene (human), a transmembrane channel-like protein coding gene; siRNA design generally targets exonic mRNA regions to promote Ago2/RISC-mediated cleavage of the complementary transcript.
  • Physical form: Supplied lyophilized or in solution by some vendors; specific form not specified for this item.

• 2D structural description (literature)

  • Each duplex consists of two single-stranded ribonucleotides forming Watson–Crick base pairs (A:U, G:C) along a ~19 bp core with 3′ overhangs. Sugar-phosphate backbone contains repeating phosphodiester linkages with negatively charged phosphate groups at physiological pH.

Synthetic Utility

• Applicability

  • This product is a biological oligonucleotide reagent (siRNA), not a small-molecule building block or reagent for organic synthesis.

• Brief guidance

  • Not typically used in chemical synthesis workflows or as a substrate/reagent in named organic reactions. Instead, it serves as a functional genomics tool for transient gene silencing in cells and certain model systems.

• Recommendation

  • For chemical biology conjugations (e.g., fluorophore or biotin labeling), specialized modified siRNAs are required. No such modifications are specified for this item.

Target Specificity

• Item-specific (from Product Name)

  • Target gene: TMC2 (human).
  • Reagent type: Pre-designed siRNA Set A (multiple duplexes typically targeting distinct regions; exact number and sequences not specified for this item).

• Not specified for this item; refer to CoA/Spec Sheet

  • Individual siRNA sequences and target exons/regions.
  • Off-target assessment metrics, seed region design details.
  • Validated cell lines or tissues.
  • Any chemical modifications or strand phosphorylation states.