Tryptic Soy Agar (TSA) used for the enumeration of aerobic bacteria and the detection of settling microorganisms in pharmaceuticals and biological products.
Principle and Purpose: Tryptic digest of casein and papain digest of soybean meal supply nitrogen sources, vitamins, growth factors and other nutrients. Sodium chloride maintains balanced osmotic pressure. Agar acts as the solidifying agent.
Preparation Method: Weigh 40.0 g of this product and add to 1 L of purified water. Heat and stir until completely dissolved. Sterilize by autoclaving at 121 °C for 15 min. Cool to 50–55 °C and pour into petri dishes for later use.
Scope of Application:
| Application | Category |
| Non-selective count | General-purpose |
Formula (g/L):
| Ingredient | Content |
| Tryptic digest of casein (Tryptone) | 15.0 g |
| Papaic digest of soybean meal | 5.0 g |
| Sodium chloride | 5.0 g |
| Agar | 15.0 g |
| Final pH (at 25°C) | 7.3 ± 0.2 |
Microbiological Testing:
Test in accordance with the Chinese Pharmacopoeia: Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans and Aspergillus niger.
| QC Strain & ATCC/CMCC No. | Growth Recovery | Growth / Colonial Morphology |
| Staphylococcus aureus CMCC(B) 26003 | 0.5 – 2.0 | Golden-yellow colonies |
| Pseudomonas aeruginosa CMCC(B) 10104 | 0.5 – 2.0 | Yellow-green colonies, diffusible green pigment |
| Bacillus subtilis CMCC(B) 63501 | 0.5 – 2.0 | Grayish-white irregular colonies |
| Candida albicans CMCC(F) 98001 | 0.5 – 2.0 | Milky white colonies |
| Aspergillus niger CMCC(F) 98003 | 0.5 – 2.0 | White mycelium with black conidia |
Inoculation level: 10–100 CFU.
Incubation conditions: Bacteria: 30–35 °C for 3 days; Fungi: 30–35 °C for 5 days.
Reference Standard:
Chinese Pharmacopoeia
Precautions
1. Take appropriate protective measures when weighing the medium to avoid physical discomfort.
2. Store the medium in a dark and dry place. Tighten the cap immediately after use to prevent moisture absorption and caking.
3. Discard used medium after autoclaving at 121 °C for 30 minutes.
4. This product is not intended for clinical testing.
