UCHL1 Mouse mAb - Anticuerpo primario, specific to Human UCHL1

Cat. No.: Ab183834

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG2b

Clone Number

AB02/2F6

Conjugation

Unconjugated

Descripción general

Mouse anti Human UCHL1 Antibody, clone AB02/2F6, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Specifications

Product Name

UCHL1 Mouse mAb - Anticuerpo primario, specific to Human UCHL1

Sinónimos

Epididymis luminal protein 117 antibody | Epididymis secretory protein Li 53 antibody | HEL 117 antibody | HEL S 53 antibody | NDGOA antibody | Neuron cytoplasmic protein 9.5 antibody | OTTHUMP00000218137 antibody | OTTHUMP00000218139 antibody | OTTHUMP00

Especificaciones y pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis. Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR,

Especies hospedadoras

Ratón

Especificidad

Human UCHL1

Inmunógeno

Recombinant protein corresponding to Human UCHL1

Control positivo

WB: HEK293, A549 and SK-N-SH whole cell lysates Flow Cytometry: SH-SY5Y cells IF/ICC: HeLa cells

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150.0 KDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Western Blot: UCHL1 Mouse mAb (Ab183834)

UCHL1 Mouse mAb (Ab183834) - Western Blot
All lanes: UCHL1 Mouse Antibody (Ab183834) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 37.9 s

Flow Cytometry: UCHL1 Mouse mAb (Ab183834)

UCHL1 Mouse mAb (Ab183834) - Flow Cytometry ​
Flow Cytometry analysis of HeLa cells stained with UCHL1  Mouse mAb (Ab183834) (Red) at 0.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue).​

ICC/IF: UCHL1 Mouse mAb (Ab183834)

UCHL1 Mouse mAb (Ab183834) - ICC/IF
IF analysis of UCHL1 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with UCHL1 Mouse mAb (Ab183834) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  1/200;

• WB:

  1/1000 - 1/200000;

• Flow Cytometry:

  1/1000 - 1/250000;

Objetivos asociados (humanos)

UCHL1 Tchem Ubiquitin carboxyl-terminal hydrolase isozyme L1 (0 Activities)

Discover Target: UCHL1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (UCHL1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.