Urease (URH), CAS No.9002-13-5

CAS: 9002-13-5 Cat. No.: U1492281 Número EC: 232-656-0

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, >150 U/mg protein; expressed in E.coli

Accession #

Q03282,Q03283,Q03284

Expression system

E. coli

Bioactivity

>150 U/mg protein

Descripción general

Ureases, functionally, belong to the superfamily of amidohydrolases and phosphotriesterases. It is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Reaction Equation

Specification
Specific Activity: >150 U/mg protein
Contaminants:
NADPH oxidase: <0.001%
Properties
pH stability: pH 8.0-9.5 (37°C, 1 weeks)
Thermal stability: ≤ 65°C (pH 8.0, 10 min.)
Optimum pH: 6.0
Optimum temp.: ≥37°C
Km value: 1.94× 10⁻⁵ mmol/L (Urea)
Molecular weight: 60.3 kDa  α subunit, 11.7 kDa β subunit, 11.1 kDa λ subunit (SDS-PAGE)
Assay Procedure
I Spectrophotometric Method
Wavelength: 340 nm, Light path length: 1 cm
Temperature: 25°C
Pipette the following reagents into a cuvette:
3.00 mL: Triethanolamine-HCl buffer (0.1 mol/L, pH 7.0) containing Urea (1 mol/L)、α-Ketoglutarate (5 mmol/L)、NADPH (0.24 mmol/L)、GIDH (20 U/mL)
0.02 mL: rUrease (approx. 1.5 U/mL)
II Calculation

ΔA/min: The change in absorbance at 340 nm per minute
V: Total volume of reaction mixture (3.02 mL)
D: Enzyme dilution factor
6.2: mmol/L extinction coefficient of NADPH (L⋅mmol −1⋅cm −1)
d: Light path length (1 cm)
v: Volume of enzyme sample (0.02 mL)
Reference Data

Specifications

Product Name

Urease (URH), CAS No.9002-13-5

Sinónimos

ureA | ureB | ureC

Grado

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Especificaciones y pureza

EnzymoPure™, ActiBioPure™, Recombinant, Bioactive, High Performance, >150 U/mg protein; expressed in E.coli

Bioactividad

>150 U/mg protein

Número de adhesión

Q03282,Q03283,Q03284

Peso molecular previsto

60.3 kDa α subunit, 11.7 kDa β subunit, 11.1 kDa λ subunit (SDS-PAGE)

CAS

9002-13-5

Número de la Comisión de Enzimas

EC 3.5.1.5

Tipo de molécula

Enzima

Almacenamiento y envío

Concentración

>150 U/mg protein; expressed in E.coli

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20℃ long term.Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Preguntas frecuentes

What does Bioactive mean?

Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 9002-13-5.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.