Urine Glucose Qualitative Detection Kit (Modified Benedict's Method) - BioReagent

Cat. No.: U1509394

GRADE & PURITY BioReagent

Synonyms

Urine Glucose Qualitative Kit (Modified Benedict's Method)

Storage

Room temperature

Shipped In

Normal

Overview

Benedict's reagent, also known as Benedict's solution, Benedict's qualitative reagent, or Benedict's test solution, is a light blue solution. This kit uses a modified Benedict's reagent for detection. The principle is that glucose, containing an aldehyde group, can reduce the blue Cu²⁺ in the solution to a yellow or brick-red precipitate of cuprous oxide (Cu₂O) under conditions of high heat and strong alkalinity. It is primarily used for the qualitative detection of glucose content in human or animal urine. This kit is for research use only and is not suitable for clinical diagnosis or other purposes.

Operating Steps (For Reference Only)

1. Place 1 mL of the Urine Glucose Qualitative Reagent in a test tube and heat to boiling for 1 minute; it should not change color. Then add 0.1 mL of a 0.5% glucose solution; a positive reaction should occur. Proceed with the following test if these conditions are met; otherwise, discard the reagent.

2. Place 1 mL of the Urine Glucose Qualitative Reagent in a test tube, then add 0.1 mL of fresh urine. Boil for 1–2 minutes and observe the results after cooling.

Result Interpretation

Result Description	Interpretation	Glucose Content (mmol/L)
The reagent shows no color change; a blue turbidity may appear if high levels of phosphates are present.	Negative (–)	0.1～1
Appears green after cooling, but without precipitate.	Doubtful (±)	<6
Appears yellow-green and turbid, with a small amount of yellow precipitate at the tube bottom.	Positive (+)	6～28
Appears green and turbid after 1 minute of boiling, with considerable yellow precipitate.	Positive (2+)	28～55
Appears ochre-colored precipitate after boiling for 15 seconds.	Positive (3+)	55～110
Appears heavily brick-red and turbid immediately upon boiling, precipitating rapidly, with a colorless supernatant.	Positive (4+)	>110

Reference Interval

Negative (glucose content 0.1 – 1 mmol/L). Glycosuria may occur when blood glucose exceeds the renal threshold (> 8.88 mmol/L).

Precautions

1. When heating over an alcohol burner, ensure boiling is maintained for no less than 1 minute.

2. Allow the mixture to cool naturally after boiling; do not cool it rapidly with cold water.

3. If the urine contains a large amount of urates, place the sample in a refrigerator, allow salts to settle, and use the supernatant for testing.

4. If the urine contains a large amount of ammonium salts, add excess alkali and boil for several minutes before performing the test.

5. Strong reducing substances may cause false positives: Vitamin C, traditional Chinese medicines containing reducing components (e.g., rhubarb, coptis, forsythia), lactose/fructose (non‑glucose reducing sugars), cysteine, sodium thiosulfate, etc. High concentrations of certain metabolites may interfere: chloral hydrate metabolites, ketone bodies (in high ketonuria, e.g., diabetic ketoacidosis), large amounts of urates, etc.

6. For your safety and health, please wear a lab coat and disposable gloves during operation.

7. Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.

Notes

Specifications

Synonyms

Urine Glucose Qualitative Kit (Modified Benedict's Method)

Specifications & Purity

BioReagent

Stability And Storage

Store at room temperature long term (12 months).

Storage

Room temperature

Shipped In

Normal

Grade

BioReagent