Experiments on the preparation and dissection of tetrads of Saccharomyces cerevisiae
A standard light microscope is required for yeast tetrad analysis, and the microscope stage should be able to be moved along the x and y axes and the distance t
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Yeast cell nucleus preparation experiment
The nucleus (nucleus) is the largest and most important organelle in the cell (the old junior high school textbooks believe that the nucleus is not an organelle
Yeast cell plasmid isolation experiment
Under non-selective growth conditions, most E. coli/yeast shuttle vectors are lost at a frequency of about 1%, and one plasmid that is not easily isolated and r
Tissue and embryo fixation and embedding experiments
The role of fixation, from the histological point of view its simple definition is to maintain the intrinsic morphology and structure of cells and tissues, so t
Handling experiments on pre-coated slides
Slides coated with polylysine (a Thermo product) with durable bioadhesion for attaching frozen and paraffin-embedded sections that can be subsequently centrifug
Yeast protoplast preparation experiment
Protoplasts are formed by the differentiation of protoplasm, specifically the cell membrane and intramembranous cytoplasm and other life-active organelles. In p
Slicing experiments on samples in wax blocks
Paraffin section is the most widely used method in the routine histology technique. Paraffin section is not only used to observe the morphology and structure of
Yeast vector and expression product detection experiments
Because of the simplicity and sensitivity of the β-galactosidase assay, yeast genes are frequently labeled with the functional portion of the lacZ gene to indi
Frozen section fixation experiment
This experiment is mainly used for fixing frozen sections.