nuclear transcription analysis
Nuclear runoff (runoff) analysis can be used to examine the transcriptional efficiency of specific genes in isolated cell nuclei. For runoff analysis, the nucle
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RNA reverse transcription
The RNA template is used to obtain DNA by reverse transcription, which is then used to replicate and infect cells.
RNA radiolabeling
Intramolecular labeling of RNA during transcription, post-transcriptional labeling of the 5' end of RNA using T4 polynucleotide kinase and labeling of the 3' en
Nuclease cleaves RNA at a specific site
Nuclease is a useful tool for obtaining site-specific cleavage products of long fragments of RNA for the study of RNA modification, for modification site analys
Deoxyribonuclease cleaves RNA at a specific site
Deoxyribonucleases are oligodeoxyribonucleotides consisting of about three dozen deoxyribonucleotides, which are easy to prepare and convenient to use, and are
An in vitro translation system for African Xenopus oocytes
The African Xenopus oocyte in vitro translation system is capable of sustained modification and processing of most exogenous mRNAs due to its ability to translo
Translation of mRNA in reticulocyte lysates
The mRNA extracted from mammalian cells or produced by in vitro transcription of cloned DNA can be translated in cell-free extracts to synthesize proteins, whic
mRNA 3' end processing in vitro
There are two strategies for in vitro experiments; one is to co-incubate a plasmid containing the target gene at the Po ly( A) locus with cell-free extracts to
Translation of mRNA in wheat germ extracts
Wheat germ extract in vitro translation system for efficient translation of mRNA in viruses and eukaryotic cells in a heterogeneous cell-free system