Translation of mRNA in reticulocyte lysates
The mRNA extracted from mammalian cells or produced by in vitro transcription of cloned DNA can be translated in cell-free extracts to synthesize proteins, whic
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Extracellular system for translating viral mRNA
Translation of poliovirus and hepatitis C virus mRNA with mammalian cell S10 extracts.
Methylmercury hydroxide agarose gel electrophoresis
Methylmercury hydroxide reacts with the imine bonds on uracil and guanine in RNA, which are involved in Watson-Crick base pairing. Therefore, methylmercury hydr
Formaldehyde denaturing agarose gel electrophoresis
Agarose gel electrophoresis is commonly used to isolate RNA prior to Northern blotting.
RNA spectroscopy and quantification
Nucleic acids are accurately quantified using spectrophotometry because this method does not destroy the structure and also allows for sample recovery. RNA has
RNA SI Nuclease Mapping
SI nuclease is an endonuclease that was isolated from Aspergillus oryzae. It can degrade single-stranded nucleic acids but not double-stranded nucleic acids. In
Glyoxal/DMSO denaturing agarose gel electrophoresis
Under slightly acidic conditions, the two acetaldehyde groups of glyoxal interact with the imino group of guanosine to form a cyclic compound, which inhibits th
Primer extension method RNA analysis
The primer extension method can be used for RNA mapping and RNA 5' halo. In this method, an excess of a 5' end-labeled single-stranded RNA primer is hybridized
A rapid response system for coupling in vitro transcription with in vitro translation
TNT 耦联网织红细胞裂解物系统乂可以分为：TNTT7 系统、TNTSP6 系统和 TN 下 PCR 系统。 The TNTT7 system translates proteins from both linear and circular DNA, the S