Total RNA or poIy(A) +RNA from Drosophila embryos
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Analysis of RNA-peptide interactions by PACE
The polyacrylamide coelectrophoresis (PACE ) assay is a relatively recently developed method for quantitatively analyzing the interaction of proteins and peptid
Determination of RNA-protein dissociation constants by nitrocellulose filter membrane binding assays
The binding of proteins to DNA and RNA can be determined using nitrocellulose membrane binding, which is based on the fact that most proteins bind to nitrocellu
Northwestern screening of protein expression library assay
Northwestern screening of protein expression libraries is performed by constructing IPTG-inducible fusion protein expression libraries (the N-terminus of the fu
RNaseH cleavage protection experiments for oligonucleotide targeting in RNA- protein complexes
Oligonucleotide-targeted RNase II digestion assay is a method to analyze RNA protein complexes (RNP, ribonucleoprotein particles), both for crude extracts and p
Liquid-phase screening of RNA-binding proteins in cDNA expression libraries
The method of immobilizing cDNA library-expressed proteins on nitrocellulose or nylon membranes and then screening them with labeled RNA is the most commonly us
Large-scale preparation of Drosophila RNA
This method allows for the preparation of RNA from 100 Drosophila or approximately 2g of Drosophila embryos.
Yeast three-hybrid system for detecting and analyzing RNA-protein interactions
The yeast three-hybrid system can be used to analyze protein-RNA interactions. In the yeast three-hybrid system, RNA-protein interactions lead to transcription
Gel blocking assay to analyze RNA-protein interaction constant assay
The simplicity of the gel reiardadon assay (also known as mobthty shift assay) method, its sensitivity, and its ability to be quantified have made it widely use