RNA interference technology
Post-transcriptional gene silencing (PTGS) is known as RNA interference (RNAi), whereby double-stranded RNA (dsRNA) consisting of positive-sense and negative-se
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Psoralen-mediated photocrosslinking reaction to study the interaction between RNA and protein molecules experimentally
An RNA-protein molecular complex analysis method based on the photochemical reaction of psoralen enables the analysis of the topological conformation of RNA-pro
shRNA Design
Design Short Hair Clip RNA
Determination of the amount of DNA fixed in ethanol
Determination of DNA content can be used to (1) perform gene fragment ligation experiments. (2) Perform plasmid concentration determination prior to transformat
denaturing gradient gel electrophoresis
Denaturing gradient gel electrophoresis (DGGE) is a gel system that enables the separation of DNA fragments according to their melting properties. (1) By settin
DNA Sequence Analysis Technology
DNA sequence analysis techniques can be used to (1) analyze the genetic diversity of species, (2) identify new species, and (3) for comparative genomics.
Subcloning of target genes
Subcloning of the target gene can be applied to (1) further analysis of the target gene and (2) gene recombination.
Single Nucleotide Polymorphism (SNP) Assay
SNP (Single Nucleotide Polymorphism), or Single Nucleotide Polymorphism, can be used to detect nucleic acid sequence polymorphisms (Polymorphism) due to changes
Plasmid preparation
Plasmid preparation can be used for (1) carrying exogenous genes into bacteria for amplification or expression of the main vector, which has an important role i