Preparation of radiolabeled DNA probes using the polymerase chain reaction
The source of this experiment is "Guide to Molecular Cloning Experiments, Third Edition", translated by Huang Peitang et al.
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Labeling of DNA by random primer method
Using oligonucleotides as primers, DNA polymerase can initiate DNA synthesis along a single-stranded template (Gotilian 1969). If the sequences of the oligonucl
Series of experiments for analyzing gene expression
SAGE (Velculescuetal.1995) is a highly sensitive method based on PCR technology to study gene expression and to obtain qualitative and quantitative information
Construction and screening of cDNA libraries-Experimentation of methods for identification of newly expressed genes under external environmental stress conditions
Materials Many organisms are excellent models for disease studies or for exploring the molecular adaptive mechanisms that enable cells and organisms to cope wit
Intracellular injection of mRNA
Modern Neuroscience Research Techniques Author(s): U. Windhorst & H. Johansson Translated by Zhao Zhiqi Chen Jun
Combined with magnetic bead experiments
This step is only part of Experiment A in the cDNA synthesis for serial analysis of gene expression. In Experiment B, the cDNA is already bound to the streptavi
Electron microscope in situ hybridization technique experiment
Electron microscopy in situ hybridization experiments can be used to (1) study the subcellular localization of specific nucleotide sequences; (2) many researche
Metabolic labeling experiments at isolated synapses
mRNA and rRNA are present within dendrites and axons (VanMinnen 1994; Steward 1997). It is puzzling whether mRNAs located in regions outside the cytosol are act
Experiments on in situ hybridization techniques for cultured neurons
The purpose of in situ hybridization (ISH) is to visualize TKmRNA molecules within cellular structures. High temperatures are used in the ISH procedure, which p