Electron microscope in situ hybridization technique experiment
Electron microscopy in situ hybridization experiments can be used to (1) study the subcellular localization of specific nucleotide sequences; (2) many researche
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BAC/PAC library construction
BAC (Bacterial Artificial Chromosome) libraries can be used for (1) whole genome sequencing; (2) construction of physical maps, chromosome step-by-step; (3) gen
Experiments on the application of the comet assay to environmental toxicology
The comet assay, also known as the single-cell gel electrophoresis assay, is a method used to detect D N A damage in almost all nucleated cells. The comet assay
FISH Basic Techniques and Problem Solving
Fluorescence in situ hybridization (FISH) technology allows the detection of specific nucleic acid sequences in chromosomes, cells and tissues. The unambiguous
Color Tape Experiment
The interspecies color banding technique is a simple and rapid method for detecting chromosomal abnormalities that cannot be identified by G-banding. This techn
Interphase FISH study experiments in chronic myeloid leukemia
The purpose of this chapter is to apply fluorescence in situ hybridization to the study of interphase nuclei in patients with chronic myeloid leukemia (CML). In
Chromosome Microdissection Experiment
It has been well known in recent decades that chromosomal rearrangements are present in most human tumors and in certain specific human genetic diseases. Tumor-
Experiments in primer-mediated in situ labeling techniques
Primed in situ labeling (PRINS) is a hybridization technique that stains cells and tissues for specific DNA sequences, and it is capable of obtaining the same t
Multicolor fluorescence in situ hybridization assay
M-FISH has been successfully used to identify marker or derived chromosomes in congenital diseases. Recently, this method has been increasingly used to identify