Urea gradient polyacrylamide gel electrophoresis and folding analysis experiments
Goldenberg and Creighfn (1984) introduced the use of polyacrylamide gels containing a concentration gradient of urea to analyze the folded state of proteins. A
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Trypsin digestion assay of calmodulin
This experiment describes the trypsin digestion process of calmodulin. This experiment was derived from Protein Purification and Identification Laboratory Guide
Experiments on the fragmentation of Escherichia coli cells and preparation of inclusion bodies
This experiment describes the fragmentation of E. coli cells and the preparation of inclusion bodies. This experiment is from the Laboratory Guide for Protein P
Experiments on the preparation of nuclear extracts from HeLa cells
In this experiment, nuclear extracts were prepared from HeLa cells essentially as described by Dignam et al. (1983). This basic method is probably the most comm
Preparation of DNA affinity media
This experiment describes the preparation process of DNA affinity medium. This experiment is from the Laboratory Guide for Protein Purification and Characteriza
Operational experiments in affinity chromatography
This experiment introduces the related operation of affinity chromatography. This experiment is from the Laboratory Guide for Protein Purification and Identific
Quantitative measurement of proteins
There is a great need for a rapid and accurate method for quantitatively determining the protein concentration in a sample so that one can follow the recovery o
Enzyme assay experiment
In this unit, a simpler method was used to determine its stimulatory effect on core RNA polymerase transcription from a non-specific template poly(dAT) start. T
Gel mobility change analysis experiment
In this experiment, we used the same complementary oligonucleotide used for DNA affinity chromatography (see the end of the Introduction to Experiment 3 for the