Measurement of protein concentration in the presence of interfering substances
Protein concentrations have traditionally been determined by the method of Lowry and co-workers (Lowry et al., 1951) using the Folin-Ciocalteau reagent, but thi
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Protein binding assay with immunoaffinity mediators
This experiment describes how much time it takes for a protein to bind to an immunoaffinity medium. This experiment is from the Protein Purification and Identif
Inclusion body precipitation dissolution assay
This experiment describes the amount of sodium N-dodecyl sarcosinate required for solubilizing inclusion body precipitation. This experiment is from the Laborat
Purity estimation experiment by overloaded stained SDS gel scanning method
Since proteins can be separated with high resolution on SDS gels and sensitive staining can be given with Caumas Brilliant Blue (CBB), the purity of the protein
An alternative experimental approach to the isolation of crude plasma membrane fractions from tissues
The method described by Neville (1968) works well for isolating murine liver plasma membranes, but is not necessarily applicable for isolating plasma membranes
Evaluation of homogeneity experiments by nondenaturing gel electrophoresis mobility shift rate method
When a protein is electrophoresed under conditions that keep its natural conformation constant, its mobility can be used as a measure of its homogeneity. Since
New purification experiments of bacterial overexpression products (without disulfide bonds)
The purpose of this section is to provide an abbreviated set of working procedures for how to use a series of experiments similar to those described in this uni
Rapid protein spot blotting assay
In routine protein spot blotting, the blotting membrane is closed for 1 hour, reacted with primary antibody for 1 hour, and then reacted with secondary antibody
Screening experiments of descaling agents for solubilizing membrane proteins
Although TriumX-100 is a good descaling agent for solubilizing insulin receptors from murine liver plasma membranes, it may not be suitable for solubilization o