Peptide isolation and purification experiments for protein internal sequence analysis
One of the most important aspects of molecular biology is the analysis of trace proteins, making possible the cloning of cDNA sequences encoding the proteins to
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3228 articles
Autophosphorylation experiments on insulin receptor stimulated by insulin
In this experiment, we will examine the insulin-stimulated tyrosine kinase activity of the insulin receptor. The phosphorylated receptor will be detected with a
Acidified acetone/methanol precipitation method experiment
Another useful alternative to TCA precipitation is the use of an acidified acetone-methanol mixture. Acidification helps to remove SDS from the sample by dissol
Cross-linking experiments of the insulin receptor with [125I]insulin
This experiment describes about the cross-linking process of insulin receptor with [125I] insulin. This experiment is from the Experimental Guide for Protein Pu
Coupling experiments of ligands with activation media
Here, we introduce this procedure by taking the coupling of ligand to Affi-Gel10 as an example. This experiment was derived from Protein Purification and Identi
Determination of isoelectric point of proteins by isoelectric focusing electrophoresis
Isoelectric focusing (IEF for short) is a new technique that appeared in the mid-sixties. In recent years, IEF has made new progress and has rapidly developed i
Design and synthesis experiments of artificial zinc finger proteins
Among DNA-binding modulators, (Cys)2(His)2-type zinc-finger modulators have a large potential for manipulation. Zinc finger modulators provide an attractive fra
Experiments on design and optimization techniques for coiled spiral structures
Despite their apparent simplicity, coiled coil (Coiled Coil) modules are highly specialized and are important in understanding tertiary structure and its format
Design and screening of protein libraries - a probabilistic calculation experiment
When designing protein libraries for screening, we must do everything possible to generate a diversity of protein variants within the physical limits of what ca