An experiment to determine the osmotic potential of plant tissues
Plant tissue into a series of increasing concentrations of sucrose or mannitol solution, after a certain period of time to achieve osmotic equilibrium, the cell
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Experiments on the fluorescence phenomenon of chlorophyll
Matter has different energy states, some electrons in the matter absorbed the energy of light quanta, the matter from the original stable state of the energy le
Catalase activity assay experiment
H2O2 is strongly absorbed at 240 nm, and catalase decomposes hydrogen peroxide, causing the absorbance of the reaction solution (A240) to decrease with reaction
Chlorophyll saponification assay
Chlorophyll is an ester of a dicarboxylic acid that saponifies with a base to form a basic salt of chlorophyllic acid; after the formation of the salt, chloroph
Experimental morphological observations on vesicles in young wheat roots
The tip of the germinating wheat embryonic root was cut (about 1 cm from the tip) and immersed in neutral red solution for live staining for 10-15 min, then rem
Experiments on the determination of photosynthetic intensity of plants
According to the similarity of the structure and physiological function of the two sides of the leaf midvein, you can first cut off half of the leaf in the dark
Experiments on oxidation and phosphorylation in isolated mitochondria
When sufficient oxygen is supplied to plant tissues, plant cells can oxidize substrates completely. When glucose is fully oxidized as a respiratory substrate, C
Experiments on the absorption spectra of chlorophyll and carotenoids
Because chlorophyll and carotenoids have different molecular structures, they have different absorption spectra. Carotenoids absorb blue-violet light and chloro
Experiments for the quantitative determination of chlorophyll
According to the absorption spectrum of chlorophyll on visible light, the use of spectrophotometer at a particular wavelength to determine its optical density,