ARF6 Antibody - Primary antibody, specific to ARF6, Rabbit IgG

Cat. No.: Ab089477

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Cat, Human, Mouse, Pig, Rat, Zebrafish

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human ARF6 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function：
GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development (By similarity). Contributes to the regulation of dendritic branching and filopodia extension.

Specifications

Product Name

ARF6 Antibody - Primary antibody, specific to ARF6, Rabbit IgG

Synonyms

ADP ribosylation factor protein 6 antibody | ADP-ribosylation factor 6 antibody | ARF6 antibody | ARF6_HUMAN antibody | DKFZp564M0264 antibody | Small GTP binding protein antibody | Small GTPase antibody | ADP ribosylation factor 6 antibody

Specifications & Purity

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Host species

Rabbit

Specificity

ARF6

Immunogen

A synthetic peptide derived from human ARF6

Positive Control

WB: Mouse liver, HeLa, MCF7, Hep G2, MDA-MB-231 and C6 lysates. IHC: Human lung cancer tissue. IF/ICC: C6 cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: ARF6 Antibody (Ab089477)

ARF6 Antibody (Ab089477) - Western Blot
All lanes: ARF6 Antibody (Ab089477) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 20 kDa
Observed band size: 18, 95 kDa
Exposure time: 25.0 s

IF/ICC: ARF6 Antibody (Ab089477)

ARF6 Antibody (Ab089477) - IF/ICC
Immunofluorescent analysis of ARF6 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ARF6 Antibody (Ab089477) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin -AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

IHC: ARF6 Antibody (Ab089477)

ARF6 Antibody (Ab089477) - IHC
Immunohistochemical analysis of ARF6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ARF6 Antibody (Ab089477) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Application

• Application: Dilution info
• IF/ICC:

  1/50-1/200 ;

• IHC:

  1/50 - 1/100;

• WB:

  1/500-1/1000 ;

Associated Targets(Human)

ARF6 Tbio ADP-ribosylation factor 6 (0 Activities)

Discover Target: ARF6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action