Determine the necessary mass, volume, or concentration for preparing a solution.

Donkey Anti-Rabbit IgG H&L (Biotin) (Ab136895)- ELISA
Donkey Anti-Rabbit IgG H&L (Biotin) (Ab136895) was tested by direct ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215) (0.5μg/mL,0.1μg/mL and uncoated control). A serial dilution of Donkey Anti-Rabbit IgG H&L (Biotin) (Ab136895) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

