GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.See COA
Carrier Free, ExactAb™, Azide Free, Validated, See COA Azide Free,Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Rabbit anti Human ARPC4 Antibody, Polyclonal, could be used for WB, IHC and so on. Application:
WB: 0.01-2µg/mL IHC: 5-20µg/mL
IF/ICC: 2.3µg/mL Protein Function Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
Specifications
Product Name
ARPC4 Antibody - Anticorpo primario, specific to ARPC4, Rabbit IgG
Sinonimi
Actin related protein 2/3 complex subunit 4 20 kD antibody | Actin related protein 2/3 complex subunit 4 20kDa antibody | Actin related protein 2/3 complex, 20-KD subunit antibody | Actin-related protein 2/3 complex subunit 4 antibody | ARC20 antibody | A
Specifiche e purezza
Carrier Free, ExactAb™, Azide Free, Validated, See COA
Specie ospite
Coniglio
Specificità
ARPC4
Immunogeno
Recombinant ARPC4 expressed in E.coli (AA 3-165)
Controllo positivo
WB: HeLa, Jurkat, HEK293, THP-1, NIH/3T3 and C6 cell lysates. IHC: Human liver tissue. IF/ICC: HeLa cells.
Coniugazione
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Metodo di purificazione
Antigen affinity purified + Protein A purified
Concentrazione
See COA
Stoccaggio e spedizione
Forma
Liquid
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini
ARPC4 Antibody (Ab089682) - Western Blot All lanes: ARPC4 Antibody (Ab089682) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa Observed band size: 19 kDa Exposure time: 5.0 s
ARPC4 Antibody (Ab089682) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 20 µg/mL ARPC4 Antibody (Ab089682) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARPC4 Antibody (Ab089682) - IHC DAB staining on IHC Sample: Human Liver Tissue Primary Ab: 20 µg/mL ARPC4 Antibody (Ab089682) Control: Used PBS instead of primary antibody Second Ab: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARPC4 Antibody (Ab089682) - IF/ICC IF analysis of ARPC4 Antibody (Ab089682) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ARPC4 Antibody (Ab089682) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Applicazione
Applicazione
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IF/ICC
2.3µg/mL
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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