beta Actin Mouse mAb (Biotin) - Anticorpo primario, specific to beta actin, IgG1

Cat. No.: Ab175850

GRADE & PURITY ExactAb™, Azide Free, Validated, High Performance, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

consiglio, Mouse, Umano

Isotype

Mouse IgG1

Clone Number

FG01/2D10

Conjugation

Biotin

Panoramica

Application:
WB :1:5000-1:40000
IF: 1:2000
Flow: 1:500-1:2500

Specifications

Product Name

beta Actin Mouse mAb (Biotin) - Anticorpo primario, specific to beta actin, IgG1

Sinonimi

A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | Beta cytoskeletal

Specifiche e purezza

ExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL

Specie ospite

Mouse

Specificità

beta actin

Immunogeno

Synthetic peptide in beta Actin

Controllo positivo

WB: K562, A549, HeLa, Jurkat, NIH3T3 and C6 whole cell lysates Flow: Hela IF: Hela

Coniugazione

Biotin

Grado

Azide Free, ExactAb™, High Performance, Validated

Isotipo

IgG1

Metodo di purificazione

Protein A purified

Concentrazione

1.0 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light,Do not freeze

Spedito in

Wet ice,Do not freeze

Stabilità e conservazione

Store at 4℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Immagini

IF: beta Actin Mouse mAb (Biotin) (Ab175850)

beta Actin Mouse mAb (Biotin) (Ab175850) - IF
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Biotin) (Ab175850) at 1/2000 dilution in blocking buffer overnight at 4°C. Then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Western Blot: beta Actin Mouse mAb (Biotin) (Ab175850)

beta Actin Mouse mAb (Biotin) (Ab175850) - Western Blot
All lanes: beta Actin Mouse mAb (Biotin) (Ab175850) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
SA-HRP: 1:20000

Predicted band size: 42.0 kDa
Observed band size: 42.0 kDa
Exposure time: 12.1s

Flow Cytometry: beta Actin Mouse mAb (Biotin) (Ab175850)

beta Actin Mouse mAb (Biotin) (Ab175850) - Flow Cytometry
Flow cytometry analysis of Beta Actin in Hela cells (red) compared to an isotype control (blue). The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Biotin) (Ab175850) at 1/100 dilution for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/2000 for 30 minutes at 4°C in the dark.

Applicazione

Applicazione

Dilution info

Flow Cytometry

1:500-1:2500

WB

1/5000 - 1/40000

IF/ICC

1:2000

Obiettivi associati (umani)

TUBB Tclin Tubulin beta chain (0 Activities)

Discover Target: TUBB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

ACTB Tbio Actin, cytoplasmic 1 (0 Activities)

Discover Target: ACTB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

TUBB Tclin Tubulin beta-5 chain (37 Activities)

Discover Target: TUBB

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (beta Actin Mouse mAb (Biotin)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.