beta Actin Mouse mAb (HRP) - Anticorpo primario, specific to beta actin, IgG1

Cat. No.: Ab175849

GRADE & PURITY ExactAb™, Azide Free, Validated, High Performance, 0.5 mg/mL

Application

WB

Host species

Mouse

Reactive species

consiglio, Mouse, Umano

Isotype

Mouse IgG1

Clone Number

FG01/2D10

Conjugation

HRP

Panoramica

Application:
WB :1:2000-1:10000

Specifications

Product Name

beta Actin Mouse mAb (HRP) - Anticorpo primario, specific to beta actin, IgG1

Sinonimi

A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | Beta cytoskeletal

Specifiche e purezza

ExactAb™, High Performance, Validated, Azide Free, 0.5 mg/mL

Specie ospite

Mouse

Specificità

beta actin

Immunogeno

Synthetic peptide in beta Actin

Controllo positivo

WB: K562, A549, HeLa, Jurkat, NIH3T3 and C6 whole cell lysates

Coniugazione

HRP

Grado

Azide Free, ExactAb™, High Performance, Validated

Isotipo

IgG1

Tipo di catena leggera

kappa

Metodo di purificazione

Protein A purified

Concentrazione

0.5 mg/mL

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Immagini

Western Blot: beta Actin Mouse mAb (HRP) (Ab175849)

beta Actin Mouse mAb (HRP) (Ab175849) - Western Blot
All lanes: beta Actin Mouse mAb (HRP) (Ab175849) at 1/5000 dilution
Samples: Lysates at 20 µg per lane

Predicted band size: 42.0 kDa
Observed band size: 42.0 kDa
Exposure time: 41.8s

Applicazione

Applicazione

Dilution info

WB

1/2000 - 1/10000

Obiettivi associati (umani)

ACTB Tbio Actin, cytoplasmic 1 (0 Activities)

Discover Target: ACTB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

How should this product be stored?

Store at ?20 °C, protected from light. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (beta Actin Mouse mAb (HRP)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.