QUALITÀ & PUREZZACarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Rabbit anti Human CCT2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-3 μg/mL
IHC: 5-30 μg/mL
IF/ICC: 2.5 μg/mL
Protein Function
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Specifiche
Product Name
CCT2 Antibody - Anticorpo primario, specific to CCT2, Rabbit IgG
Sinonimi
99D8.1 antibody|CCT 2 antibody|CCT beta antibody|CCT-beta antibody|CCT2 antibody|CCTB antibody|Chaperonin containing t complex polypeptide 1 beta subunit antibody|Chaperonin containing t complex polypeptide 1 subunit 2 antibody|Chaperonin containing TCP1 subunit 2 antibody|Chaperonin containing TCP1 subunit 2 (beta) antibody|CTP:phosphocholine cytidylyltransferase 2 antibody|Epididymis secretory sperm binding protein Li 100n antibody|HEL S 100n antibody|MGC142074 antibody|MGC142076 antibody|MGC94480 antibody|PRO1633 antibody|T complex protein 1 beta subunit antibody|T complex protein 1 subunit beta antibody|T-complex protein 1 subunit beta antibody|TCP 1 beta antibody|TCP-1-beta antibody|CCTB|CCT-beta|TCP-1-beta
Specifiche e purezza
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Specie ospite
Coniglio
Specificità
CCT2
Immunogeno
Recombinant CCT2 expressed in E.coli (AA 1-488).
Controllo positivo
WB: HeLa and MCF7 cell lysates. IHC: Human glioma, kidney, stomach, breast cancer, cerebrum, spleen and Porcine cerebrum tissues. IF/ICC: HeLa cells.
Coniugazione
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Metodo di purificazione
Antigen affinity purified + Protein A purified
Concentrazione
See COA
Product Line
Prodotti biologici
Stoccaggio e spedizione
Forma
Liquid
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini
CCT2 Antibody (Ab093602) - Western Blot All lanes: CCT2 Antibody (Ab093602) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 57 kDa Observed band size: 54 kDa Exposure time: 12.6 s
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Glioma Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Samples: Porcine Cerebrum Tissue Primary Ab: 20 μg/mL CCT2 Antibody (Ab093602) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Sample: Human Cerebrum Tissue Primary Ab: 20 µg/mL CCT2 Antibody (Ab093602) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Sample: Human Spleen Tissue Primary Ab: 20 µg/mL CCT2 Antibody (Ab093602) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IHC DAB staining on IHC Sample: Human Breast cancer Tissue Primary Ab: 30 µg/mL CCT2 Antibody (Ab093602) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
CCT2 Antibody (Ab093602) - IF/ICC
IF analysis of CCT2 Antibody (Ab093602) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CCT2 Antibody (Ab093602) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Applicazione
Applicazione
Dilution info
IHC
5-30 µg/mL
WB
0.01-3 µg/mL
IF/ICC
2.5 µg/mL
Dati avanzati
Obiettivi associati (umani)
CCT2TbioT-complex protein 1 subunit beta (0 Activities)
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Recensioni
Recensioni dei clienti
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