CD14 Mouse mAb - Anticorpo primario, specific to CD14, Mouse IgG2a

Cat. No.: Ab093878

GRADE & PURITY Carrier Free, Low Endotoxin, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Flow Cytometry, IF/ICC, IHC, IP, WB

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG2a

Clone Number

UCHM-1

Conjugation

Unconjugated

Panoramica

Mouse anti Human CD14 Antibody, Monoclonal (UCHM-1), could be used for Flow, WB, IHC, IF, ICC, IP and so on.
Application
Flow: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
IF/ICC: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
Protein Function
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Specifications

Product Name

CD14 Mouse mAb - Anticorpo primario, specific to CD14, Mouse IgG2a

Sinonimi

CD14 antigen | CD14 molecule | CD14 | monocyte differentiation antigen CD14 | Myeloid cell-specific leucine-rich glycoprotein | CD 14 antibody | CD_antigen=CD14 antibody | CD14 antibody | CD14 antigen antibody | CD14 molecule antibody | CD14_HUMAN antibod

Specifiche e purezza

Carrier Free,Low Endotoxin,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Specie ospite

Mouse

Specificità

CD14

Immunogeno

Human Thymocytes followed by peripheral blood mononuclear cells

Coniugazione

Unconjugated

Grado

Azide Free, Carrier Free, Low Endotoxin, PBS Only, Validated

Isotipo

Mouse IgG2a

Tipo di catena leggera

kappa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Flow Cytometry: CD14 Mouse mAb (Ab093878)

CD14 Mouse mAb (Ab093878) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD14 with CD14 Mouse mAb (Ab093878) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD14 Mouse mAb (Ab093878)

CD14 Mouse mAb (Ab093878) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD14 (red) with CD14 Mouse mAb (Ab093878) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

Applicazione

Dilution info

WB

Use at an assay dependent concentration.

IP

Use at an assay dependent concentration.

IHC

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

IF/ICC

Use at an assay dependent concentration.

Obiettivi associati (umani)

CD14 Tbio Monocyte differentiation antigen CD14 (0 Activities)

Discover Target: CD14

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Low Endotoxin mean?

Low Endotoxin indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD14 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.