Topic

Western blot

Browse protocols, FAQs, guidelines, and technical articles tagged “Western blot”, newest first. For research use only.

82 resources · protocols, FAQs, guidelines & articles
Protocol

PRCP Antibody Protocol: WB, IHC & IF Applications

Step-by-step PRCP Antibody protocol for Western blot, IHC, and immunofluorescence. Optimize detection of prolylcarboxypeptidase with Ab349917 from Aladdin Scientific.

Updated ·5 min read
Protocol

PKC-beta 1 Mouse mAb: Protocol & Usage Guide

Step-by-step protocol for PKC-beta 1 Mouse mAb (Ab349200): recommended dilutions, sample prep, WB, IHC, and storage conditions for optimal results.

Updated ·6 min read
Protocol

ADAM9 Mouse mAb: Protocol & Application Guide

Step-by-step protocol for ADAM9 Mouse mAb (Ab351140): optimal dilutions, sample prep, storage, and troubleshooting for WB, IHC, and IF applications.

Updated ·5 min read
Article

Immunoprecipitation Kits: Principles, System Composition, and Typical Applications

Immunoprecipitation (IP) is a classical biochemical method that enables selective enrichment of target proteins through antibody–antigen specific recognition. It is commonly used to obtain target-protein inputs with a higher signal-to-noise ratio from complex lysates, supporting Western ...

Updated ·10 min read
Article

Western Blot

The success of an experiment relies on high-quality sample preparation and precise, reliable quantitative analysis. To support your protein research, Aladdin has meticulously developed a series of reagents and kits tailored to critical steps of protein workflows. From accurate protein ...

Updated ·5 min read
Protocol

Western Blot Protocol

Protein blotting (WB) is widely used to analyze the expression of specific proteins in cell or tissue extracts. Western blot experiments are time-consuming, and their success has a significant impact on research progress.

Updated ·10 min read
Protocol

Immobilized IP Protocol

Immunoprecipitation (IP) experiments are important techniques in biochemistry and molecular biology for studying protein interactions and protein expression status.

Updated ·3 min read
Protocol

General Immunoprecipitation Protocol

Immunoprecipitation (IP) is a molecular biology technique based on antigen-antibody specific binding, which is used to selectively separate and enrich target proteins from complex biological samples. The core of this technology is to capture the protein of interest using specific antibodies on a ...

Updated ·7 min read
Article

Introduction to the principles related to the immunoblotting experiment

Western blot is used to identify large molecular weight antigens (usually proteins) that can interact with specific antibodies and to determine the size of the antigen. Proteins are first separated by SDS-PAGE and then transferred by electrophoresis to a solid support, which includes ...

Updated ·4 min read
Protocol

Protocol of Western blot for Phosphorylated Proteins

Phosphoprotein western blot is a widely used technique for detecting and analyzing the phosphorylation status of proteins, which is essential for understanding various biological processes and signal transduction pathways. Some common application scenarios include signal transduction research, ...

Updated ·3 min read
Protocol

Histone Western Blot

The basic principles and operation steps of Western Blot experiments of histones are similar to those of other conventional proteins, both of which rely on specific antibodies to detect the presence and expression of target proteins. However, due to the special properties and functions of ...

Updated ·4 min read
Article

Principles of Western Blot Internal Reference Antibody Selection

There are many kinds of Western Blot internal reference antibodies, such as β-actin, β-tubulin, GAPDH, Lamin B, etc., so how should we do the selection for our own experiments? Below is a brief introduction to the principles that should be followed in selecting internal reference antibodies.

Updated ·3 min read
Article

Method and Precautions of SDS-PAGE Gel Preparation

SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a commonly used technique to separate proteins based on size. By denaturating the protein and giving it a uniform negative charge, the protein migrates to the positive pole under the application of an electric field and is ...

Updated ·5 min read

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