CD46 Mouse mAb - Anticorpo primario, specific to Human CD46

Cat. No.: Ab210969

GRADE & PURITY Animal Free, Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

AB03/5B11

Conjugation

Unconjugated

Panoramica

Mouse anti Human CD46 Antibody, clone AB03/5B11, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Specifications

Product Name

CD46 Mouse mAb - Anticorpo primario, specific to Human CD46

Sinonimi

AHUS2 antibody | Antigen defined by monoclonal antibody TRA 2 10 antibody | Antigen identified by monoclonal antibody TRA 2 10 antibody | CD46 antibody | CD46 antigen antibody | CD46 antigen complement regulatory protein antibody | CD46 molecule antibody

Specifiche e purezza

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Meccanismi biochimici e fisiologici

Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fert

Specie ospite

Mouse

Specificità

Human CD46

Immunogeno

Recombinant protein corresponding to Human CD46

Controllo positivo

WB: HeLa, K562, A431, A549 and MCF-7 whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells

Coniugazione

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo di catena leggera

kappa

SDS-PAGE

150.0 KDa

Metodo di purificazione

Protein A purified

Concentrazione

1.0 mg/mL

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Immagini

Flow Cytometry: CD46 Mouse mAb (Ab210969)

CD46 Mouse mAb (Ab210969) - Flow Cytometry 
Flow Cytometry analysis of HeLa cells stained with CD46 Mouse mAb (Ab210969) (Red) at 0.125 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG1 was used as an isotype control (Blue).

Flow Cytometry: CD46 Mouse mAb (Ab210969)

CD46 Mouse mAb (Ab210969) - Flow Cytometry 
Flow Cytometry analysis of HeLa cells stained with CD46 Mouse mAb (Ab210969) (Red) at 0.125 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG1 was used as an isotype control (Blue).

Flow Cytometry: CD46 Mouse mAb (Ab210969)

CD46 Mouse mAb (Ab210969) - Flow Cytometry 
Flow Cytometry analysis of HeLa cells stained with CD46 Mouse mAb (Ab210969) (Red) at 0.0625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG1 was used as an isotype control (Blue).​

ICC/IF: CD46 Mouse mAb (Ab210969)

CD46 Mouse mAb (Ab210969) - ICC/IF
IF analysis of CD46 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with CD46 Mouse mAb (Ab210969) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Applicazione

• Applicazione: Dilution info
• WB:

  1/1000 - 1/2000

• IF/ICC:

  1/200;

• Flow Cytometry:

  1/1000 - 1/50000

Obiettivi associati (umani)

CD46 Tbio Membrane cofactor protein (0 Activities)

Discover Target: CD46

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD46 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.