ExactAb™, Validated, Carrier Free, 0.5mg/mL Carrier Free,ExactAb™,Validated per flussi di lavoro cromatografici e analitici sensibili che richiedono un'interferenza minima della linea di base.
Store at -20°C,Avoid repeated freezing and thawing Spedizione Ice chest + Ice pads. Consultare il COA specifico del lotto per le specifiche esatte.
SDS, COA, scheda tecnica e scheda delle specifiche disponibili per il download. COA specifico del lotto accessibile tramite ricerca per numero di lotto.
Citato in 0 pubblicazioni peer-reviewed su cromatografia, sintesi organica e reazioni di accoppiamento incrociato.
Mouse anti human CDK5 Antibody, Monoclonal (1552CT262.105.8), could be used for WB, IHC, Flow, ICC, IF and so on.
Applications
WB: 1/2000
IHC: 1/100-1/500
IF/ICC: 1/25
Flow：1/25
Function
Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
CDK5 Mouse mAb (Ab095769) - Western Blot
All lanes: CDK5 Mouse mAb (Ab095769) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution
Predicted band size: 33 kDa
Observed band size: 30 kDa
Exposure time: 11.9 s
CDK5 Mouse mAb (Ab095769) - ICC/IF
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling CDK5 with CDK5 Mouse mAb (Ab095769) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
CDK5 Mouse mAb (Ab095769) - IHC
CDK5 Mouse mAb (Ab095769) staining CDK5 in human skeletal muscle sections by Immunohistochemistry (paraformaldehyde - fixed, paraffin - embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
CDK5 Mouse mAb (Ab095769) - Flow Cytometry
Overlay histogram showing K562 cells stained with CDK5 Mouse mAb (Ab095769) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by CDK5 Mouse mAb (Ab095769) (1/25 dilution) for 60 min at 37ºC. The secondary antibody was used Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 used under the same conditions. Acquisition of >10000 events was performed.
1/100-1/500
1/25
1/2000
1/25
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Visualizza scheda delle specifiche →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Data | Oggetto |
|---|---|---|---|
 ZJ24F1011871 | Certificate of Analysis | Aug 11, 2026 | Ab095769 |
| ZJ23F1001319 | Certificate of Analysis | Aug 07, 2025 | Ab095769 |
| ZJ23F1001320 | Certificate of Analysis | Aug 07, 2025 | Ab095769 |
| ZJ26F0635968 | Certificate of Analysis | Aug 07, 2025 | Ab095769 |
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