Deoxyribonuclease I, CAS No.9003-98-9

CAS: 9003-98-9 Cat. No.: D128598 Numero EC: 232-667-0

GRADE & PURITY EnzymoPure™, ≥2,000 Kunitz units/mg dry weight;Filtered;from Bovine Pancreas

Accession #

P00639

Panoramica

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.

Specifications

Product Name

Deoxyribonuclease I, CAS No.9003-98-9

Sinonimi

Deoxyribonucleate 5′-oligonucleotido-hydrolase;DNase I

Grado

EnzymoPure™

Specifiche e purezza

EnzymoPure™, ≥2,000 Kunitz units/mg dry weight;Filtered;from Bovine Pancreas

Meccanismi biochimici e fisiologici

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both

Numero d'ordine

P00639

CAS

9003-98-9

Numero della Commissione Enzimi

3.1.21.1

Tipo di molecola

Enzima

Stoccaggio e spedizione

Concentrazione

≥2,000 Kunitz units/mg dry weight;Filtered;from Bovine Pancreas

Condizioni di conservazione di stoccaggio

Store at 2-8°C

Spedito in

Wet ice

Definizione dell'unità

1 Unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0. Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Worthingto