Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
In bottom-up proteomics, the digestion step determines peptide-length distribution, accessibility of cleavage sites, peptide physicochemical properties, and subsequent mass-spectrometric detectability. It is therefore a key upstream determinant of proteome coverage depth and quantitative ...
Omics systematically characterizes, in a high-throughput manner, the molecular composition and dynamics of biological systems across DNA, RNA, proteins, and metabolites, giving rise to core branches including genomics, transcriptomics, proteomics, and metabolomics. In plant research, omics is ...
Proteases are enzymes that catalyze the hydrolysis of peptide bonds in proteins or polypeptides. In life-science research, they are widely used for proteomics digestion, limited proteolysis for domain mapping, deproteinization of nucleic-acid samples, antibody fragmentation, and ...
Mass spectrometry (MS)-based proteomics has become an essential tool for biologists over the last decade. The ability of a mass spectrometer to identify thousands of proteins from a complex biological sample has revolutionized scientific experiments.
Techniques often explored alongside immunological experiments.
