DNAJC3 Antibody - Primary antibody, specific to DNAJC3, Rabbit IgG

Cat. No.: Ab100175

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Cow, Human, Mouse, Pig, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human DNAJC3 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.

Specifications

Product Name

DNAJC3 Antibody - Primary antibody, specific to DNAJC3, Rabbit IgG

Synonyms

DnaJ (Hsp40) homolog subfamily C member 3 antibody | DnaJ homolog subfamily C member 3 antibody | DNAJC3 antibody | DNJC3_HUMAN antibody | double-stranded RNA-activated protein kinase inhibitor antibody | Endoplasmic reticulum DnaJ protein 6 antibody | ER

Specifications & Purity

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Biochemical and Physiological Mechanisms

Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein

Host species

Rabbit

Specificity

DNAJC3

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DnaJC3.

Positive Control

WB: COLO 205 and Raji cell lysates. IHC: Human colorectal cancer tissue. IF/ICC: SGC7901 cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: DNAJC3 Antibody (Ab100175)

DNAJC3 Antibody (Ab100175) - Western Blot
All lanes: DNAJC3 Antibody (Ab100175) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 57 kDa
Exposure time: 3.7 s

Western Blot: DNAJC3 Antibody (Ab100175)

DNAJC3 Antibody (Ab100175) - Western Blot
All lanes: DNAJC3 Antibody (Ab100175) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 57 kDa
Exposure time: 3.7 s

IHC: DNAJC3 Antibody (Ab100175)

DNAJC3 Antibody (Ab100175) - IHC
Immunohistochemical analysis of DNAJC3 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with DNAJC3 Antibody (Ab100175) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: DNAJC3 Antibody (Ab100175)

DNAJC3 Antibody (Ab100175) - IF/ICC
Immunofluorescent analysis of DNAJC3 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with DNAJC3 Antibody (Ab100175) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/50 - 1/200;

Associated Targets(Human)

DNAJC3 Tbio DnaJ homolog subfamily C member 3 (0 Activities)

Discover Target: DNAJC3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)