DNAJC3 Antibody - Anticorpo primario, specific to DNAJC3, Rabbit IgG

Cat. No.: Ab100175

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Coniglio

Reactive species

consiglio, Maiale, Mouse, Mucca, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human DNAJC3 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.

Specifications

Product Name

DNAJC3 Antibody - Anticorpo primario, specific to DNAJC3, Rabbit IgG

Sinonimi

DnaJ (Hsp40) homolog subfamily C member 3 antibody | DnaJ homolog subfamily C member 3 antibody | DNAJC3 antibody | DNJC3_HUMAN antibody | double-stranded RNA-activated protein kinase inhibitor antibody | Endoplasmic reticulum DnaJ protein 6 antibody | ER

Specifiche e purezza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Meccanismi biochimici e fisiologici

Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein

Specie ospite

Coniglio

Specificità

DNAJC3

Immunogeno

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DnaJC3.

Controllo positivo

WB: COLO 205 and Raji cell lysates. IHC: Human colorectal cancer tissue. IF/ICC: SGC7901 cells.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Immunogen affinity purified

Concentrazione

1.0 mg/mL

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: DNAJC3 Antibody (Ab100175)

DNAJC3 Antibody (Ab100175) - Western Blot
All lanes: DNAJC3 Antibody (Ab100175) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 57 kDa
Exposure time: 3.7 s

Western Blot: DNAJC3 Antibody (Ab100175)

DNAJC3 Antibody (Ab100175) - Western Blot
All lanes: DNAJC3 Antibody (Ab100175) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 57 kDa
Exposure time: 3.7 s

IHC: DNAJC3 Antibody (Ab100175)

DNAJC3 Antibody (Ab100175) - IHC
Immunohistochemical analysis of DNAJC3 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with DNAJC3 Antibody (Ab100175) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: DNAJC3 Antibody (Ab100175)

DNAJC3 Antibody (Ab100175) - IF/ICC
Immunofluorescent analysis of DNAJC3 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with DNAJC3 Antibody (Ab100175) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Applicazione

• Applicazione: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/50 - 1/200;

Obiettivi associati (umani)

DNAJC3 Tbio DnaJ homolog subfamily C member 3 (0 Activities)

Discover Target: DNAJC3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (DNAJC3 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.