QUALITÀ & PUREZZACarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
Rabbit anti Human EXOSC9 Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC9 binds to ARE-containing RNAs.
Specifiche
Product Name
EXOSC9 Antibody - Anticorpo primario, specific to EXOSC9, Rabbit IgG
A synthetic peptide derived from human EXOSC9 (AA 392-426).
Controllo positivo
WB: K562 and Hep G2 cell lysates. IHC: Human gastric cancer tissue.
Coniugazione
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
Metodo di purificazione
Antigen affinity purified
Concentrazione
1.0 mg/mL
Product Line
Prodotti biologici
Stoccaggio e spedizione
Forma
Liquid
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini
EXOSC9 Antibody (Ab102506) - Western Blot All lanes: EXOSC9 Antibody (Ab102506) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 49 kDa Observed band size: 65 kDa Exposure time: 19.9 s
EXOSC9 Antibody (Ab102506) - Western Blot All lanes: EXOSC9 Antibody (Ab102506) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 49 kDa Observed band size: 65 kDa Exposure time: 19.9 s
EXOSC9 Antibody (Ab102506) - IHC Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using EXOSC9 Antibody (Ab102506). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Certificati (CoA, COO, BSE/TSE e tabella di analisi)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Calcolatori di soluzioni
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Recensioni
Recensioni dei clienti
Application Protocols
Item-specific tested applications, dilutions, and positive controls: Not specified for this item; refer to CoA/Spec Sheet.
General starting points (literature guidance; not item-specific recommendations)
Western blot: Start at 1:500–1:2,000 for unconjugated primaries; adjust based on signal. Load 10–40 µg total protein per lane.
Immunofluorescence/ICC: Start at 1:100–1:500; optimize fixation (PFA 2–4%) and permeabilization (0.1–0.3% Triton X-100) as needed.
IHC: Begin at 1:100; evaluate antigen retrieval (citrate pH 6 or Tris-EDTA pH 9). Use appropriate detection system.
IP/Co-IP: 1–5 µg antibody per mg of lysate protein as a common range; titrate to minimize nonspecific binding.
ELISA (capture/detection): 0.5–2 µg/mL coating or 0.1–1 µg/mL detection, depending on format and plate.
Controls and validation (general)
Include isotype controls, no-primary controls, and known positive/negative biological samples. For specificity assessment, use CRISPR/Cas9 or siRNA knockdown models where feasible.
Note
ExactAb™/Validated designations indicate internal QC was performed, but the precise protocol parameters for this SKU are not provided here.
Biological Roles
General background on the target (EXOSC9) — literature context, not item-specific
EXOSC9 (Exosome Component 9) is a core subunit of the multi-protein RNA exosome complex, a 3′→5′ exoribonuclease assembly that mediates processing and degradation of a broad range of RNA substrates in eukaryotic cells.
Functional context: The exosome participates in maturation of rRNA, snRNA, and snoRNA, quality control of aberrant transcripts, and turnover of mRNA. EXOSC9 contributes to scaffold/structural integrity and recruitment within the exosome core ring.
Localization: Predominantly nuclear for many exosome functions, with roles also described in cytoplasmic RNA decay pathways (literature).
Interactions: EXOSC9 associates with other exosome core components (e.g., EXOSC1–8, EXOSC10/RRP6, DIS3/RRP44) and adaptor complexes that specify RNA targets (literature).
Research utility: Monitoring EXOSC9 expression and localization provides insight into RNA metabolism, gene expression regulation, and RNA surveillance mechanisms in diverse biological systems.
Notes
The above describes the biology of the antigen; it does not constitute performance information for this specific antibody. For validated applications and sample types, refer to the CoA/Spec Sheet.
Buffer Applications
Item-specific facts (from Product Data)
Supplied formulation and recommended diluents: Not specified for this item; refer to CoA/Spec Sheet.
General buffer guidance for antibody use (not item-specific)
Stock handling: Maintain in a neutral pH buffer (e.g., PBS pH 7.4). If carrier-free, consider adding stabilizers only to working solutions when compatible with the assay.
Working buffers by application:
WB: TBS-T or PBS-T (0.05–0.1% Tween-20). Blocking with 3–5% BSA or non-fat dry milk depending on detection system.
IF/ICC: PBS with 1–5% BSA or serum, 0.1% Tween-20 only if permeabilization is needed; for permeabilization use 0.1–0.3% Triton X-100 or saponin as appropriate.
IP/Co-IP: Non-denaturing lysis buffers (e.g., 20–50 mM Tris, 150 mM NaCl, 1% NP-40 or Triton X-100) with protease/RNase inhibitors; adjust salt and detergent to preserve protein complexes.
IHC: Citrate (pH ~6.0) or Tris-EDTA (pH ~9.0) for heat-induced epitope retrieval as required by tissue fixation.
Filtration/sterility: Use 0.22 µm low-binding filters if sterility is necessary; avoid repeated filtration to limit loss.
Note
Optimize buffer composition empirically for EXOSC9 detection in your specific samples; consult the CoA/Spec Sheet for any item-specific recommendations.
Green Alternatives
For antibody-based workflows, environmental impact is more about buffer selection, plastic use, and energy, rather than solvent substitution.
Practical greener choices (general, not item-specific)
Buffer systems: Use phosphate- or Tris-based buffers prepared from concentrated stocks to reduce packaging waste. Avoid azide in waste streams when possible; collect separately if used.
Conjugation chemistries: Prefer aqueous, click-like bioconjugations (e.g., SPAAC) that avoid organic solvents, when compatible.
Energy and cold chain: Optimize freezer usage by aliquoting to reduce door-open time; consolidate shipments and use recyclable cold packs when feasible.
Consumables: Choose low-binding, recyclable plastics where validated; consider glass where appropriate to reduce adsorption for non-carrier formulations.
Trade-offs
Addition of stabilizers (e.g., glycerol) can improve longevity but complicate waste and some assays. Balance stability with downstream compatibility.
Sodium azide preserves sterility but complicates disposal and can inhibit HRP; evaluate need relative to sterile technique and intended detection system.
Pharmaceutical Uses
This product is for research use only, as stated in the Product Data. No clinical or therapeutic use is implied or permitted.
General formulation roles for antibodies in non-clinical settings (not item-specific)
Conjugate precursor: Carrier-free antibodies are suitable starting materials for creating research-grade conjugates (enzymes, fluorophores, biotin) using NHS ester, maleimide, or click-chemistry linkers.
Analytical controls: Use as positive controls or reference reagents in bioanalytical method development (e.g., ligand-binding assays) when appropriate.
Regulatory note
No pharmacopeial status is claimed. For any regulated application, lot-specific documentation (CoA, SDS) and internal qualification are required.
Restriction
For research use only. Not for human or animal diagnostic or therapeutic use.
Physical Properties
Item-specific facts (from Product Data)
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Concentration/formulation: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
General/literature values (not item-specific)
Typical whole IgG molecular mass: ~150 kDa (literature), with Fc glycosylation causing microheterogeneity.
Isoelectric point (pI): Often between ~6–9 depending on isotype and sequence (literature).
Solubility: Antibodies are soluble in aqueous buffers (e.g., PBS, TBS) with modest ionic strength; stability can be enhanced with protein stabilizers (e.g., 0.1–1% carrier protein) and cryoprotectants (e.g., 10–50% glycerol) when compatible with the intended assay (literature).
Stability considerations: Sensitive to repeated freeze–thaw cycles; prolonged exposure to elevated temperature or extreme pH promotes aggregation and loss of binding affinity (literature).
Practical notes
Exact extinction coefficient (A280) and concentration should be taken from the label/CoA if provided.
If preparing working solutions, filter through low protein-binding membranes (e.g., 0.22 µm PVDF with caution; many prefer no filtration for antibodies unless bioburden control is required) to minimize loss by adsorption.
Quality and Grades
Item-specific facts (from Product Data)
Grade/Purity: Carrier Free; ExactAb™; Validated.
What these designations typically imply (general guidance; not item-specific specifications)
Carrier Free: Supplied without protein carriers (e.g., BSA, gelatin). Advantages include lower background in immunoassays, compatibility with conjugation chemistries (e.g., NHS/SMCC labeling), and defined mass balance. Handle carefully to minimize adsorption losses (use low-binding tubes, include inert protein only at the point-of-use if necessary).
ExactAb™ (platform descriptor): Generally denotes a well-defined antibody format (often recombinant or sequence-defined), aiming for lot-to-lot consistency, controlled glycosylation or expression system, and consistent binding. Verify exact meaning and format on the CoA/Spec Sheet.
Validated: Indicates the antibody has undergone application-appropriate verification. However, specific validated applications, species reactivity, and performance metrics are not provided here. Consult the CoA/Spec Sheet for the exact validation matrix, recommended dilutions, and positive controls.
Quality considerations and controls
Lot-to-lot consistency: For critical assays, record lot numbers and perform incoming QC (e.g., test on known positive control lysate or tissue).
Purity: Carrier-free preparations may be highly pure by SDS-PAGE/SEC; confirm by CoA if required for regulated workflows.
Endotoxin/bioburden: If relevant (e.g., cell-based assays), check CoA for limits. Not specified for this item.
Reaction and Applications
This section is not about chemical synthesis reactions for small molecules; for antibodies, “applications” refer to immunoassays and affinity workflows.
Item-specific facts (from Product Data)
Validated grade is indicated, but specific tested applications are not provided. Refer to the CoA/Spec Sheet for verified application list and performance data.
General/literature applications for an EXOSC9-targeting antibody (not item-specific)
Western blot (WB): Detection of EXOSC9 in cell/tissue lysates to assess expression levels, often under reducing SDS-PAGE conditions.
Immunofluorescence (IF) / Immunocytochemistry (ICC): Subcellular localization studies; EXOSC9 is associated with the RNA exosome complex in the nucleus/cytoplasm depending on context.
Immunoprecipitation (IP)/Co-IP: Enrichment of EXOSC9 and associated exosome components for downstream MS or western analysis.
ELISA: Capture/detection in sandwich or indirect formats if appropriate controls and standards are established.
Practical tips (general)
Antigen retrieval for IHC may be required depending on fixation; optimize buffer (citrate pH ~6 or Tris-EDTA pH ~9) and retrieval time.
For IP/Co-IP, maintain gentle lysis (e.g., NP-40/nonionic detergents) to preserve protein–protein interactions within the exosome complex.
Include appropriate positive controls (cells known to express EXOSC9) and negative controls (isotype control, knockout/siRNA samples) to establish specificity.
Reaction Conditions
Classical reaction conditions (solvent, temperature, catalysts) do not apply to antibody use. Instead, consider assay conditions for immunoapplications.
General assay condition guidance (not item-specific)
Western blot: Reducing SDS-PAGE (e.g., 4–12% Bis-Tris), transfer to PVDF/nitrocellulose, block 3–5% BSA or milk, primary incubation 1–16 h at 4–25°C, secondary HRP-conjugate 1 h, chemiluminescent detection.
Immunofluorescence: Fix (PFA 2–4%), permeabilize as required, block with serum/BSA, primary 1–16 h at 4–25°C, fluorophore-conjugate secondary 1 h, mount with antifade medium; image under appropriate excitation/emission.
IP/Co-IP: Gentle lysis, pre-clear lysate, incubate antibody with lysate 1–4 h, capture with Protein A/G beads, wash with low-detergent buffer, elute under denaturing conditions or low pH with immediate neutralization.
Expected outcomes (literature)
Signal-to-noise is highly dependent on antibody affinity, antigen abundance, fixation, and blocking conditions. Optimize antibody amount, incubation time, and wash stringency.
Note
For item-specific recommended dilutions and validated conditions, consult the CoA/Spec Sheet. None are specified here.
Safety and Handling
Item-specific facts (from Product Data)
Storage: Store at -20°C; avoid repeated freezing and thawing.
Shipping: Ice chest + ice pads.
GHS classification, signal word, hazard statements, pictograms: Not specified for this item; refer to SDS.
General safety guidance for antibody reagents (not item-specific)
Antibodies are protein biologics typically supplied in aqueous buffers. While generally considered low hazard, always treat as laboratory reagents. Avoid inhalation of aerosols, ingestion, and contact with skin/eyes.
Personal protective equipment (PPE): Lab coat, gloves (nitrile), and safety glasses. Work in a clean area; use a biosafety cabinet if sterility must be maintained.
Incompatibilities: Avoid strong oxidizers and extremes of pH that may denature the protein. Do not mix with proteases unless intentionally digesting (e.g., papain/pepsin).
First aid overview: If skin or eye contact occurs, rinse with water for at least 15 minutes. If inhaled (aerosol), move to fresh air. If ingested, rinse mouth and seek medical attention. Consult SDS for detailed measures.
Spill/cleanup: Absorb with inert material; decontaminate with appropriate detergent; dispose according to institutional and local regulations.
Waste: Proteinaceous solutions can typically be disposed via approved liquid biological waste streams; follow institutional SOPs.
Always defer to the product-specific SDS and institutional EHS guidance for authoritative safety information.
Solvent Selection
Item-specific facts (from Product Data)
Formulation buffer and excipients: Not specified for this item; refer to CoA/Spec Sheet.
General guidance for working with antibodies (not item-specific)
Primary diluents: Use isotonic aqueous buffers such as PBS (10–50 mM phosphate, 100–150 mM NaCl, pH 7.2–7.6) or TBS (Tris-buffered saline, pH 7.4–8.0). Avoid strong acids/bases that denature proteins.
Additives: Low levels of nonionic detergents (e.g., 0.05–0.1% Tween-20) can reduce nonspecific binding in immunoassays. For storage, cryoprotectants like 10–50% glycerol are common when compatible with intended use.
Protein stabilizers: If carrier-free handling losses are a concern, consider adding inert protein (e.g., 0.1% BSA) only to working solutions when compatible with downstream applications. Do not add carriers if conjugation is planned.
Solvent incompatibilities: Organic solvents (e.g., acetone, acetonitrile, methanol at high %), high salt (>0.5–1 M), or chaotropes can destabilize or precipitate antibodies unless used for specific protocols (e.g., elution in IP at low pH followed by neutralization).
Filtration: If sterility is required, use low protein-binding filters; pre-rinse to minimize adsorption.
When to choose PBS vs TBS (literature)
PBS: Favored for cell-based assays and most immunoblots.
TBS: Preferred when phosphate can interfere (e.g., AP-conjugated systems) or when reduced background is observed in certain tissues.
Storage and Reconstitution
Item-specific facts (from Product Data)
Storage conditions: Store at -20°C; avoid repeated freezing and thawing.
Shipping: Ice chest + ice pads.
Appearance, formulation, concentration: Not specified for this item; refer to CoA/Spec Sheet.
General handling guidance (not item-specific)
Upon receipt: Centrifuge briefly to collect contents. Inspect for particulate matter; a small amount of reversible precipitate can sometimes form and may dissolve upon gentle mixing.
Aliquoting: To avoid freeze–thaw cycles, divide into single-use aliquots in low protein-binding tubes. Typical working aliquots are sized for one experiment.
Thawing: Thaw on ice; mix gently by inversion. Avoid vigorous vortexing that may denature antibodies.
Long-term storage: Many antibodies remain stable at -20°C or below; adding 10–50% glycerol can support storage at -20°C without freezing, if compatible with downstream use. For >12 months, -80°C is commonly used (literature), unless the CoA specifies otherwise.
Light/temperature: Protect from light if conjugated to fluorophores/HRP; minimize time at room temperature.
Sterility: If sterility is required, work aseptically; consider a single 0.22 µm filtration using low-binding membranes.
Documentation
For exact buffer composition, concentration, and reconstitution instructions (if lyophilized), consult the item’s CoA/Spec Sheet. Research use only.
Structure and Identity
Item-specific facts (from Product Data)
Product: EXOSC9 Antibody (SKU: Ab102506)
Grade/Purity: Carrier Free; ExactAb™; Validated
Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
Sequence/clone/isotype/host/species reactivity: Not specified for this item; refer to CoA/Spec Sheet.
CAS, InChIKey, SMILES: Not applicable to antibodies; not specified for this item.
General/literature context (not item-specific)
Antibodies are Y-shaped immunoglobulin proteins composed of two heavy and two light chains stabilized by disulfide bonds. Domain architecture typically includes variable domains (VH/VL) that bind the antigen and constant domains (CH/CL) that define isotype and effector functions.
The antigen here is EXOSC9 (Exosome Component 9), a core subunit of the RNA exosome complex involved in RNA processing/turnover.
2D depiction (conceptual): Two identical heavy chains forming the long “arms” and stem, two identical light chains forming the outer portions of the arms; antigen-binding sites located at the tips of the Fab regions; an Fc region forms the stem.
Notes
Structural identifiers used for small molecules (SMILES, InChIKey) are not meaningful for protein biologics such as antibodies.
For exact sequence, clonality (mono/polyclonal), and isotype, consult the CoA/Spec Sheet or SDS.
Synthetic Utility
Not typically applicable. Antibodies are biologic macromolecules used as affinity reagents, not as building blocks or catalysts in small-molecule synthesis.
General laboratory utility (not item-specific)
Affinity capture: Immobilize on Protein A/G or directly conjugate to solid supports for immunoaffinity purification of EXOSC9 and associated complexes.
Conjugation: Carrier-free format facilitates covalent labeling with fluorophores (e.g., Alexa dyes), enzymes (HRP/AP), or haptens (biotin) using amine- or thiol-reactive chemistries.
Fragmentation: Enzymatic generation of Fab/F(ab′)2 fragments (papain/pepsin) for applications needing smaller binders or reduced Fc interactions.
Target Specificity
Item-specific facts (from Product Data)
Antigen/Target name: EXOSC9
Not specified for this item; refer to CoA/Spec Sheet
Clonality (mono/polyclonal)
Host species / expression system
Isotype / subclass
Species reactivity (human/mouse/rat/other)
Epitope mapping or immunogen details
Cross-reactivity and blocking/neutralization data
Notes
Provide experimental controls appropriate for your system (positive control lysates/tissues known to express EXOSC9; isotype controls; knockout/knockdown validation where feasible).
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